Integrated GC-MS and LC-MS-Based Untargeted Metabolomics Reveals Diverse Metabolites in Fermented Pine Needles  

作　　者：Yinyu Xu Zheng Li Qian Wen Hao He Pengtu Shi Wenhua Zhou 

机构地区：[1]Hunan Provincial Institute of Product and Goods Quality Inspection,Changsha,410004,China [2]College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha,410004,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第9期2367-2382,共16页国际实验植物学杂志(英文)

基　　金：supported by the Hunan Provincial Market Supervision and Administration Bureau Science and Technology Plan Project(Project No.2020kJJH20);the Program for Food Safety Monitoring and Early Warning Open Project of Hunan Provincial Key Laboratory(2020KFJJ01);the Scientific Research Project of Hunan Education Department(20C1910).

摘　　要：The diversity of metabolites produced in fermented pine needles at different stages of fermentation has rarely been investigated.In the present study,untargeted metabolomic analysis using GC-MS and LC-MS was performed to detect metabolites in fermented pine needles at different fermentation stages.A total of 30 samples of pine needles fermented first with yeast(Saccharomyces cerevisiae)and then with a mixed bacterial culture of Lactobacillus fermentum CECT5716 and Bifidobacterium Breve M16V,were used to detect differential metabolites at different stages of fermentation.Pearson’s correlation analysis was used to determine correlations between metabolites and key microbial communities.A total of 708 differential metabolites(430 from LC-MS and 278 from GC-MS analysis)were identified.The PCA and OPLS-DA revealed distinct differences between metabolites at different fermentation stages.Key differential metabolites identified through GC-MS analysis included;Phosphoric acid,D-Fructose,2-O-alpha-mannosyl-D-glycerate,1,3-dihydroxyacetone dimer,Galactosylglycerol,2-Isopropylmalic acid,alpha-D-Galactose,Citrate,4-Hydroxycinnamic acid,and Shikimate.Similarly,key differential metabolites identified through LC-MS included;2-Phenlyethanol,Dimethlglycine,2-Hydroxybenzaldehyde,3-Aminoisobutanoic acid,p-Cresol,Triethylamine,2-Ketobutyric acid,Cytosine,Benzaldehyde,and Creatinine.Annotation of differential metabolites to KEGG pathway enrichment analysis revealed the association of these metabolites with phenylpropanoid,flavonoid,and secondary metabolite biosynthesis.Furthermore,the results showed that three bacterial(Firmicutes,Actinobacteria,and Lactobacillus)and three fungal genera(Penicillium,Candida,and Basidiomycota)significantly correlated with differential metabolites showing synergistic effects.Our study reveals a comprehensive comparison of metabolites at different fermentation stages and provides practical insights into the mechanism of metabolite enrichment in fermented pine needles.

关 键 词：Fermented pine needle Pinus massoniana GC-MS LC-MS metabolomics 

分 类 号：R28[医药卫生—中药学]