基于高通量测序的枇杷花药微生物多样性研究  

Assessing microbial diversity in anthers of Eriobotrya japonica(Thunb.)Lindl.based on high-throughput sequencing

作　　者：高枫 邓国翠 张伍 张野驰 龚燕兵[1] Gao Feng;Deng Guocui;Zhang Wu;Zhang Yechi;Gong Yanbing(State Key Laboratory of Hybrid Rice,Key Laboratory of Biodiversity and Environment on the Qinghai-Tibet Plateau,Ministry of Education,College of Sciences,Wuhan University,Wuhan 430072,China;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]武汉大学生命科学学院,杂交水稻全国重点实验室,青藏高原生物多样性与生态环境保护教育部重点实验室,武汉430072 [2]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《植物科学学报》2025年第1期32-40,共9页Plant Science Journal

基　　金：国家自然科学基金项目(31970249);青藏高原生物多样性与生态环境保护教育部重点实验室开放课题项目(KLBE2024012)。

摘　　要：果树具有较高的经济价值,并大多依赖传粉者实现繁殖成功以保证产量。花药微生物能影响植物的花粉活力,其组成也可能受到传粉者访问的影响;理解花药微生物的多样性和群落构建模式对于提高果树的繁殖适合度具有潜在意义,但目前相关研究仍相对缺乏。本研究基于高通量测序技术分析了传粉者访问前(套袋组)与访问后(访问组)枇杷(Eriobotrya japonica(Thunb.)Lindl.)花药中真菌与细菌的组成及差异。结果显示,传粉者访问未显著改变花药微生物的多样性及组成,套袋组中优势真菌科为枝孢菌科与球腔菌科,访问组优势真菌科则为枝孢菌科与梅奇酵母科。此外,两组中优势细菌科均为产碱杆菌科与欧文菌科。研究结果表明,枇杷花药微生物具有相对稳定的群落结构,传粉者不是驱动花药微生物群落构建的主要因素。Fruit trees,vital to global agriculture,depend heavily on pollinators to facilitate successful reproduction and ensure optimal yield.Microorganisms associated with anthers can influence pollen viability,and their community composition may be affected by pollinator activity.While understanding the diversity and community assembly patterns of these microorganisms has potential implications for enhancing the reproductive fitness of fruit trees,research in this area remains relatively scarce.This study employed high-throughput sequencing to analyze the diversity and community structure of fungi and bacteria on the anthers of loquat(Eriobotrya japonica(Thunb.)Lindl.)before(bagged group)and after(nature group)pollinator visitation.Results showed that pollinator visitation had no significant effect on the diversity or composition of anther microbiomes.In the bagged group,dominant fungal taxa included Cladosporiaceae and Mycosphaerellaceae,whereas Cladosporiaceae and Metschnikowiaceae were dominant in the nature group.Bacterial communities in both groups were also dominated by Alcaligenaceae and Erwiniaceae.These findings indicate that the microbial community composition within loquat anthers is inherently stable and largely unaffected by pollinator activity.

关 键 词：传粉者访问 花药微生物 高通量测序技术 微生物组成 

分 类 号：Q948.122.3[生物学—植物学]