续随子MYB基因家族的鉴定及ElMYB114在油脂合成中的功能分析  

Identification of MYB gene family and functional analysis of ElMYB114 in lipid synthesis of Euphorbia lathyris L.

作　　者：惠生娟 葛丽萍[1] 王子瑜 张玉胜 苏云婷 孙岩 李润植 Hui Shengjuan;Ge Liping;Wang Ziyu;Zhang Yusheng;Su Yunting;Sun Yan;Li Runzhi(College of Forestry,Shanxi Agricultural University,Taigu,Shanxi 030801,China;Shanxi Engineering Research Center for Genetics and Metabolism of Special Crops,Taigu,Shanxi 030801,China;Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Taigu,Shanxi 030801,China)

机构地区：[1]山西农业大学林学院,山西太谷030801 [2]山西省特用作物遗传和代谢工程研究中心,山西太谷030801 [3]山西农业大学分子农业与生物能源研究所,山西太谷030801

出　　处：《植物科学学报》2025年第1期92-101,共10页Plant Science Journal

基　　金：山西省自然科学基金项目(201901D111223);国家重点研发计划项目(2023YFF0805800);2022年山西省研究生教育创新项目(2022Y325)。

摘　　要：MYB转录因子广泛参与植物的代谢和种子发育等生命过程。为探究其在续随子(Euphorbia lathyris L.)油脂合成及积累过程中的调控作用,本研究对续随子MYB转录因子进行了全基因组鉴定,分析了其基因结构、编码蛋白理化性质、启动子顺式元件以及时空表达谱,并克隆了ElMYB114基因的编码序列,将其转入烟草(Nicotiana tabacum L.),进一步鉴定该基因的生物学功能。结果显示:(1)全基因组鉴定获得199个续随子MYB基因,命名为ElMYB1~ElMYB199,编码蛋白均具有SANT结构域;(2)ElMYB114编码的蛋白定位于细胞核;(3)荧光定量qRT-PCR分析结果表明,ElMYB114基因在种子发育后期的表达量最高;(4)与转空载对照相比,异源表达ElMYB114基因的烟草株系,其叶片总油脂含量分别提高了3.7%和4.2%,种子总脂含量分别提高了11.0%和10.7%,表明过表达ElMYB114基因可提高植物组织的总油脂积累。研究为深入解析MYB介导的续随子油脂生物合成调控网络奠定了基础。MYB transcription factors play pivotal roles in plant metabolism and seed development.This study conducted a comprehensive investigation into the regulatory functions of these transcription factors in lipid synthesis and accumulation in Euphorbia lathyris L..The analysis included genome-wide identification of MYB transcription factors,characterization of gene structures,physicochemical properties of encoded proteins,distribution of promoter cis-acting elements,and spatiotemporal expression patterns.The ElMYB114 coding sequence was cloned and genetically transformed into tobacco(Nicotiana tabacum L.)to characterize its biological function.Genome-wide identification revealed 199 MYB genes in E.lathyris,designated ElMYB1 to ElMYB199,all encoding proteins with SANT domains.The protein encoded by ElMYB114 was localized to the nucleus.Quantitative fluorescence qRT-PCR analysis showed that ElMYB114 expression peaked during the late stage of seed development.Overexpression of ElMYB114 in transgenic tobacco lines led to a 3.7%and4.2%increase in total oil content in leaves and an 11.0%and 10.7%increase in total lipid content in seeds compared to the empty vector control.These findings suggest that ElMYB114 enhances carbon flux within the heterologous host toward lipid synthesis pathways,thereby promoting lipid accumulation in plant tissues.This study provides foundational insights into the MYB-mediated regulatory networks governing oil biosynthesis in E.lathyris.

关 键 词：续随子 MYB转录因子 ElMYB114 油脂合成与调控 基因克隆 

分 类 号：Q943.2[生物学—植物学]