2020—2021年山东省流感样病例6种非流感病毒的核酸检测结果分析  

Analysis of the nucleic acid detection results for six non-influenza viruses in influenza-like illness cases in Shandong Province from 2020 to 2021

作　　者：何玉洁 李忠[1] 吴巨龙[1] 孙林[1] 宋绍霞[1] 张淑 刘晓林[1] 董阳 王显军[1] 寇增强[1] 刘倜[1,3,4] He Yujie;Li Zhong;Wu Julong;Sun Lin;Song Shaoxia;Zhang Shu;Liu Xiaolin;Dong Yang;Wang Xianjun;Kou Zengqiang;Liu Ti(Infectious Disease Prevention and Control Institute of Shandong Center for Disease Control and Prevention/Shandong Institute of Preventive Medicine/Shandong Provincial Key Laboratory of Intelligent Monitoring,Early Warning,Prevention and Control for Infectious Diseases(in preparation),Ji′nan 250014,China;Ji′nan Second Maternal and Child Health Care Hospital Clinical Laboratory,Ji′nan 271100,China;School of Public Health and Health Management,Shandong First Medical University,Ji′nan 250117,China;School of Public Health,Shandong Second Medical University,Weifang 261053,China)

机构地区：[1]山东省疾病预防控制中心传染病防制所,山东省预防医学研究院,山东省传染病智慧化监测预警和防控重点实验室(筹),济南250014 [2]济南市第二妇幼保健院检验科,济南271100 [3]山东第一医科大学公共卫生与健康管理学院,济南250117 [4]山东第二医科大学公共卫生学院,潍坊261053

出　　处：《中华预防医学杂志》2025年第2期216-221,共6页Chinese Journal of Preventive Medicine

基　　金：山东省自然科学基金(ZR2021MH372);山东省疾病预防控制中心青年创新基金(QC-2022-02);山东省公共卫生学会科研项目(SGWXH202301);山东省医药卫生科技项目青年项目(202312051021)。

摘　　要：目的:分析2020—2021流感监测年度山东省流感样病例中呼吸道病毒感染情况及流行病学特点。方法:根据《全国流感监测方案》(2017年版)要求,在山东省14家监测哨点医院采集就诊的流感样病例的咽拭子样本,对所有样本进行核酸提取,采用实时荧光定量PCR的方法检测偏肺病毒、副流感病毒1型、副流感病毒2型、副流感病毒3型、呼吸道合胞病毒及腺病毒等6种常见病毒,并对检测结果进行分析。结果:共收集样本2 386份,检出率为24.22%(578份);6种病毒均有检出,检出率分别为偏肺病毒6.75%(162份)、呼吸道合胞病毒5.87%(140份)、副流感病毒3型3.56%(85份)、副流感病毒2型3.14%(75份)、副流感病毒1型2.98 %(71份)和腺病毒2.77%(66份)。不同性别检出率差异无统计学意义;不同年龄组的检出率差异具有统计学意义( P<0.001),检出率最高的为0~4岁年龄组(31.94%),其次为≥60岁年龄组(26.06%)。6种病毒的流行呈现月份变化,偏肺病毒在12月份检出率较高,副流感病毒1型在2月份检出率较高,副流感病毒2型、副流感病毒3型、呼吸道合胞病毒和腺病毒均在11月份检出率较高。病毒混合检出率为0.80%,以呼吸道合胞病毒合并其他病毒感染最为常见,且混合感染主要集中在0~4岁年龄组。 结论:山东省流感样病例6种多病原检测以偏肺病毒、呼吸道合胞病毒以及副流感病毒3型为主;呼吸道病毒的流行存在年龄和时间差异。ObjectiveTo analyze the respiratory virus infection status and epidemiological characteristics of influenza-like illness(ILI)cases in Shandong Province during the 2020-2021 influenza surveillance year.MethodsAccording to the National Influenza Surveillance Plan(2017 version),throat swab samples of ILI cases were collected from 14 surveillance sentinel hospitals in Shandong Province.Nucleic acid was extracted from all samples.Real-time fluorescence quantitative PCR(RT-PCR)was utilized to detect six common viruses,including human metapneumovirus(HMPV),human parainfluenza virus(HPIV)types 1,2 and 3,respiratory syncytial virus(RSV),and adenovirus(ADV).Subsequently,the obtained detection results were analyzed.ResultsA total of 2386 specimens were collected,with a detection rate of 24.22%(578).Six viruses were detected,with detection rates of 6.75%(162 cases)for HMPV,5.87%(140 cases)for RSV,3.56%(85 cases)for HPIV3,3.14%(75 cases)for HPIV2,2.98%(71 cases)for HPIV1,and 2.77%(66 cases)for ADV.There was no significant difference in detection rates between genders,but a notable variation among different age groups(P<0.001).The highest detection rate was observed in individuals aged 0-4 years(31.94%),followed by those aged≥60 years(26.06%).The prevalence of six viruses showed a monthly variation,with the detection rate of HMPV being higher in December and HPIV1 being higher in February.HPIV2,HPIV3,RSV,and ADV had higher detection rates in November.The co-detection rate of multiple viruses was 0.80%,with RSV being the most common pathogen involved in co-detection,primarily in individuals aged 0-4 years.ConclusionThe detection of six multiple pathogens in ILI cases in Shandong Province is dominated by HMPV,RSV and HPIV3.The prevalence of respiratory viruses varies by age and time.

关 键 词：流感 人 传染病 多病原监测 

分 类 号：R511.7[医药卫生—内科学] R181.3[医药卫生—临床医学]