云南省大理白族自治州家畜片形吸虫感染情况调查及分子鉴定  

Prevalence of Fasciola infections and molecular identification of Fasciola in livestock in Dali Bai Autonomous Prefecture, Yunnan Province

作　　者：李诗韵 李慈怀 陈凤 郝明明 李新娣 赵银娇 张冬琦 张全富 李芹翠 张剑平 陈绍荣 罗天鹏 刘榆华 LI Shiyun;LI Cihuai;CHEN Feng;HAO Mingming;LI Xindi;ZHAO Yinjiao;ZHANG Dongqi;ZHANG Quanfu;LI Qincui;ZHANG Jianping;CHEN Shaorong;LUO Tianpeng;LIU Yuhua(Institute of Schistosomiasis Control in Dali Bai Autonomous Prefecture,Dali 671000,Yunnan Province,China;School of Public Health,Dali University,Dali 671000,Yunnan Province,China)

机构地区：[1]大理白族自治州血吸虫病防治研究所,云南大理671000 [2]大理大学公共卫生学院,云南大理671000

出　　处：《中国寄生虫学与寄生虫病杂志》2024年第6期715-720,共6页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家寄生虫资源库资助(NPRC?2019?194?30)。

摘　　要：目的 了解云南省大理白族自治州(以下简称大理州)家畜片形吸虫感染情况并鉴定片形吸虫种类。方法 2023年8月至2024年3月期间在大理州12个县(市)采集牛羊等家畜的粪样检查片形吸虫虫卵,每个县选取3份阳性样本的虫卵,利用显微镜成像系统拍照、测量后提取基因组DNA,PCR扩增核糖体内转录间隔区(ITS)序列,鉴定片形吸虫种类。测序序列在NCBI中进行BLAST比对分析,用MEGA11.0软件以邻接法构建系统进化树。使用SPSS 19.0软件进行统计分析,片形吸虫阳性率的比较采用χ^(2)检验。结果 共收集家畜粪样1 302份,其中牛粪样1 228份(占94.3%)、羊粪样45份(占3.5%)、马粪样18份(占1.4%)、猪粪样11份(占0.8%)。检出疑似片形吸虫虫卵阳性粪样212份,阳性率为16.3%(212/1 302)。牛、羊粪样的阳性率分别为17.2%(211/1 228)、2.2%(1/45),未检出马和猪的阳性粪样。家畜粪样阳性率由高到低依次为巍山县(45.0%,49/109)、洱源县(42.7%,44/103)、云龙县(28.2%,29/103)、弥渡县(25.4%,30/118)、宾川县(18.0%,18/100)、鹤庆县(16.5%,17/103)、祥云县(8.7%,9/103)、剑川县(6.9%,8/116)、南涧县(4.7%,5/107)、漾濞县(2.2%,3/137),大理市和永平县未检出阳性粪样。不同地区家畜片形吸虫阳性率差异有统计学意义(χ^(2)=222.975,P<0.05);雌性家畜阳性率(19.0%,165/873)高于雄性(11.0%,47/429)(χ^(2)=13.319,P<0.05)。大理州巨片形吸虫的虫卵大小为(182.30±4.35)μm×(97.80±5.11)μm,肝片形吸虫的虫卵大小为(149.49±5.90)μm×(80.46±4.37)μm。共扩增获得7条ITS序列,2条为肝片形吸虫,5条为巨片形吸虫。这7条序列均已上传至国家微生物科学数据中心数据库,获得的登录号为:宾川县A1 (NMDCN0005QTQ)、巍山县A32(NMDCN0005TN)、剑川县A70 (NMDCN0005QTO)、漾濞县A19 (NMDCN0005ELF)、鹤庆县A7 (NMDCN0005QTR)、弥渡县A35 (NMDCN0005ELH)和祥云县A18 (NMDCN0005QTP)。其中4条序列(宾川县A1、巍山县A32、剑川县A70、鹤�Objective To investigate the prevalence of Fasciola infections and to characterize the Fasciola species in livestock in Dali Bai Autonomous Prefecture.Methods Cattle,sheep,horse and pig stool samples were collected from 12 counties(city)in Dali Bai Autonomous Prefecture during the period from August 2023 to March 2024 for detection of Fasciola eggs.Parasite eggs were sampled from three Fasciola-infected stool specimens in each county,and photographed and measured with a microscopic imaging system.Then,genomic DNA was extracted from Fasciola eggs,and the ribosomal internal transcribed spacer(ITS)gene was amplified using a PCR assay for characterization of Fasciola species.The sequence of the ITS gene was aligned using the BLAST program in NCBI,and a neighbor-joining phylogenetic tree of Fasciola spp.was built based on the ITS gene sequence using the software MEGA 11.0.All statistical analyses were performed using the software SPSS 19.0,and differences in the prevalence of Fasciola infections were compared with chi-square test.Results A total of 1302 livestock stool samples were collected,including 1228 cattle stool samples(94.3%),45 sheep stool samples(3.5%),18 horse stool samples(1.4%),and 11 pig stool samples(0.8%),and there were 212 stool samples detected with suspected Fasciola eggs(16.3%).The detection of Fasciola eggs was 17.2%in cattle stool samples(211/1228)and 2.2%in sheep stool samples(1/45),and no Fasciola eggs were detected in horse or pig stool samples.The highest detection of Fasciola eggs was seen in livestock stool samples from Weishan County(45.0%,49/109),followed by from Eryuan County(42.7%,44/103),Yunlong County(28.2%,29/103),Midu County(25.4%,30/118),Binchuan County(18.0%,18/100),Heqing County(16.5%,17/103),Xiangyun County(8.7%,9/103),Jianchuan County(6.9%,8/116),Nanjian County(4.7%,5/107),Yangbi County(2.2%,3/137),and no Fasciola eggs were detected in livestock stool samples from Dali City or Yongping County.There was a region-specific prevalence of Fasciola infections in livestock in Dali Bai

关 键 词：片形吸虫 分子鉴定 家畜 大理 

分 类 号：R383.26[医药卫生—医学寄生虫学]