斑马鱼雌性在繁殖期对雄性的视觉和嗅觉性偏好  

Visual and Olfactory Preference of Zebrafish Females to Males during the Breeding Period

作　　者：符金凤 汪春玲 刘智皓[1] FU Jinfeng;WANG Chunling;LIU Zhihao(Laboratory of Water Ecological Health and Environmental Safety,College of Life Sciences,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]重庆师范大学、生命科学学院、水生态健康与环境安全研究实验室,重庆401331

出　　处：《重庆师范大学学报(自然科学版)》2024年第6期21-26,共6页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金青年科学基金项目(No.31901183);重庆市自然科学基金项目(No.cstc2020jcyj-msxmX0805);重庆市基础科学与前沿技术研究专项基金项目(No.cstc2016jcyjA1032);重庆市教育委员会科学技术研究计划项目(No.KJ1600308)。

摘　　要：探究斑马鱼(Danio rerio)雌性在繁殖期的性别偏好性以及这种性别偏好是由视觉还是嗅觉主导。设置对照组(视觉及嗅觉信号刺激)、嗅觉组(嗅觉信号刺激)和视觉组(视觉信号刺激),在斑马鱼繁殖期(8:00—10:00),将受试雌鱼放入实验装置中间启动区,而刺激鱼分别放入两侧雌、雄性刺激鱼区,对受试雌鱼的行为进行10 min视频拍摄,分析并统计各项性别偏好指标。结果显示:1)在对照组、嗅觉组和视觉组中,受试雌鱼靠近雌、雄性刺激鱼区域的停留频次均无明显差异;2)在对照组、视觉组中,受试雌鱼靠近雄性刺激鱼区域的撞壁频次、停留时间与最长停留时间明显高于它们靠近雌性刺激鱼区域的这3个指标;3)嗅觉组受试雌鱼靠近雌、雄性刺激鱼区域的撞壁频次、停留时间与最长停留时间无明显差异。研究结果提示斑马鱼雌性在繁殖期时更偏好雄性,且该性别偏好主要是由视觉主导。To explore the sex preference of zebrafish(Danio rerio)females to their mates during reproduction,the control(visual and olfactory signals stimuli),olfactory(olfactory signals stimuli)and optical(visual signals stimuli)groups were set.During reproduction(8:00—10:00),female fish were placed in the onset zone individually,and stimulus fish were placed in the male and female stimulation zone respectively.The sexual preference of zebrafish was determined for 10 minutes after light stimuli.The results showed that:1)No significant difference of the frequency in the zone was found in females of each group.2)In the control and optical groups,frequency of striking,duration in the zone and maximum of duration of the female were all significantly higher when stimulated by the males.3)In olfactory group,no significant difference of the frequency of striking,duration in the zone and maximum of duration in the female was found between sexes.The results indicated that zebrafish female significantly prefered males during reproduction and likely through an optical-triggered pathway.

关 键 词：斑马鱼 性别偏好 视觉介导 嗅觉介导 

分 类 号：Q492.5[生物学—生理学]