基于基因表达谱的黑腹果蝇组织标志基因的鉴定  

Identification of Tissue Marker Genes in Drosophila melanogaster Based on Gene Expression Profiling

作　　者：李磊 朵泓睿 李勃 李映红 郝友进[1] LI Lei;DUO Hongrui;LI Bo;LI Yinghong;HAO Youjin(College of Life Sciences,Chongqing Normal University,Chongqing 401331;School of Bioinformatics,Chongqing University of Posts and Telecommunications,Chongqing 400065,China)

机构地区：[1]重庆师范大学生命科学学院,重庆401331 [2]重庆邮电大学生物信息学院,重庆400065

出　　处：《重庆师范大学学报(自然科学版)》2024年第6期69-75,共7页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金青年科学基金项目(No.31871274);重庆市教育委员会科学技术研究计划项目(No.KJQN202100538)。

摘　　要：为解决目前黑腹果蝇(Drosophila melanogaster)组织标志基因鉴定结果不统一、不准确的问题,分别利用Fly Cell Atlas和FlyAtlas2数据库中7个共有组织的转录组学数据,通过Seurat以及计算差异表达倍数的方法鉴定果蝇组织标志基因;经过跨数据集交叉验证、文献比对和基因功能检索,最终确定准确的组织标志基因。从上述2个数据集中分别鉴定出575和5202个组织标志基因,经过跨数据集交叉验证后得到366和2671个组织标志基因,通过文献比对和FlyBase数据库基因功能检索,共得到55个核心组织标志基因和22个候选组织标志基因。建立了准确的果蝇组织标志基因集,为将来果蝇组织类型区分和细胞类型标志基因的鉴定提供了重要参考。To solve the current problem of inconsistent and inaccurate results of tissue marker gene identification in Drosophila melanogaster.Drosophila tissue marker genes were identified by Seurat as well as calculation of differential expression folds using transcriptomics data of seven shared tissues from Fly Cell Atlas and FlyAtlas2 databases,respectively.After cross-validation across datasets,literature comparison and gene function searches,accurate tissue marker genes were finally identified.From the two datasets,575 and 5202 tissue marker genes were identified,respectively,and 366 and 2671 tissue marker genes were obtained after cross-validation across the datasets,and a total of 55 core tissue marker genes and 23 candidate tissue marker genes were obtained through literature comparison and gene function search in the FlyBase database.An accurate set of Drosophila tissue marker genes was established,providing an important reference for future Drosophila tissue type differentiation and cell type marker gene identification.

关 键 词：黑腹果蝇 组织标志基因 转录组 单细胞转录组 

分 类 号：Q966[生物学—昆虫学]