阿尔茨海默病神经系统相关共病“异病同证”基因组生物信息学分析  

Bioinformatics Analysis of the Genome of Alzheimer's Disease Neurologic-related Co-morbidities"Different Disease with Same Symptome"

作　　者：杨雅丽 闵冬雨[1,2] 刘勇明[2] 梁元钰 程美佳 鞠业涛 袁常斌 何晓明[1] 张力[1] 于畅洋 YANG Yali;MIN Dongyu;LIU Yongming;LIANG Yuanyu;CHENG Meijia;JU Yetao;YUAN Changbin;HE Xiaoming;ZHANG Li;YU Changyang(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中西医结合慢性病杂志》2025年第1期18-25,共8页JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE ON CHRONIC DISEASES

基　　金：国家自然科学基金(82174114);沈阳市科学技术计划公共卫生研发专项(医工结合协同创新研究项目)(22-321-32-14);辽宁中医药大学中医脏象理论及应用教育部重点实验室开放基金项目(zyzx2006)。

摘　　要：目的通过基因组生物信息学分析探讨阿尔茨海默病(Alzheimer's disease,AD)神经系统相关共病(癫痫、睡眠剥夺、抑郁症)异病同证生物学基础。方法下载GSE63060、GSE63061、GSE140829、GSE143272、GSE98565和GSE98793数据集作为研究对象,运用韦恩工具筛选各疾病组与正常对照组之间的差异基因,运用Cytoscape软件拓扑分析筛选核心基因,运用OmicShare平台对核心基因进行基因本体论(gene ontology,GO)/京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析。结果共筛选出33个核心基因,GO富集分析显示核心基因在生物过程主要富集于细胞进程、代谢进程、生物调控、生物进程调控、刺激反应、信号传递、发育进程、多细胞生物进程、免疫系统进程、多生物进程、生殖进程、病毒进程,在细胞成分主要富集于细胞解剖实体、含蛋白复合物,在分子功能主要富集于结合途径、结构分子途径、催化活性、翻译调节活性、转录调节活性、ATP依赖性活性、分子功能调节因子、分子适配器活性、分子载体活性、分子换能器活性;KEGG富集分析显示核心基因主要富集于核糖体、高级糖基化终末产物-受体信号通路、辅助性T细胞17(T helper cell 17,Th17)细胞分化、介导细胞与细胞之间信号通路、缺氧诱导因子信号通路、程序性死亡配体1表达以及程序性死亡受体1检查点信号通路。结论运用基因组生物信息学分析探讨AD神经系统相关共病核心基因以及信号通路,能够在一定程度上揭示AD神经系统相关共病“异病同证”作用机制,对于阐述中医现代化研究以及个体化诊疗科学内涵具有重要意义。Objective To explore the biological basis of different disease with same symptome of neurologically related co-morbidities(epilepsy,sleep deprivation,depression)in Alzheimer's disease(AD)by genomic bioinformatics analysis.Methods The GSE63060,GSE63061,GSE140829,GSE143272,GSE98565 and GSE98793 datasets were downloaded as the study subjects,and the Wayne tool was used to screen the differentiated genes between each disease group and the normal control group,and Cytoscape software was used to screen the core genes by topology analysis,and the core genes were enriched by gene ontology(GO)/kyoto encyclopedia of genes and genomes(KEGG)using the OmicShare platform.Results A total of 33 core genes were screened,and the GO enrichment analysis showed that the core genes were mainly enriched in cellular processes,metabolic processes,bioregulation,bioprocess regulation,stimulus response,signaling,developmental processes,multicellular biological processes,immune system processes,multibiotic processes,reproductive processes,and viral processes,and in cellular constituents,mainly enriched in cellular anatomical entities,protein-containing complexes,and in molecular functions are mainly enriched in binding pathways,structural molecular pathways,catalytic activity,translational regulatory activity,transcriptional regulatory activity,ATP-dependent activity,molecular function regulators,molecular adapter activity,molecular carrier activity,and molecular transducer activity;KEGG enrichment analysis shows that the core genes are mainly enriched in the ribosomes,the AGE-RAGE signaling pathway,T helper cell 17(TH17)cellular differentiation,the JAK-STAT signaling pathway,the HIFG signaling pathway,and the HIFG signaling pathway,and the JAK-STAT signaling pathway.STAT signaling pathway,HIF-1 signaling pathway,PD-L1 expression,and PD-1 checkpoint signaling pathway.Conclusion The use of genome bioinformatics analysis to explore the core genes and signaling pathways of AD neurological disorders can,to a certain extent,reveal the mechanism o

关 键 词：阿尔茨海默病 神经系统相关共病 异病同证 基因组学 生物信息学 

分 类 号：R277.7[医药卫生—中医学] R749.16