库洛胡黄连实时荧光PCR鉴别方法的建立  

Establishment of a Real-Time PCR Identification Method for Picrorhiza kurroa

作　　者：周林 孙涛 滕少娜 何玲 杨登香 任凤鸣 孔德英 赵晓丽 ZHOU Lin;SUN Tao;TENG Shao-Na;HE Ling;YANG Deng-Xiang;REN Feng-Ming;KONG De-Ying;ZHAO Xiao-Li(Chongqing Customs Technology Center,Chongqing 400020;Chongqing Institute of Medicinal Plant Cultivation,Chongqing 408435;Science and Technology Research Center of China Customs,Beijing 100026)

机构地区：[1]重庆海关技术中心,重庆400020 [2]重庆市药物种植研究所,重庆408435 [3]中国海关科学技术研究中心,北京100026

出　　处：《中国口岸科学技术》2025年第1期90-96,共7页China Port Science and Technology

基　　金：中国海关科学技术研究中心自立科研课题(2023HX06)。

摘　　要：本研究通过分析比对库洛胡黄连及其近似种的叶绿体psbA-trnH序列,设计特异性引物探针,建立了一种库洛胡黄连实时荧光PCR鉴别方法。应用该方法测试29份样品的DNA,结果表明本试验所建立的实时荧光PCR方法能够快速、准确、灵敏地鉴别库洛胡黄连。A real-time PCR method for identification of Picrorhiza kurroa was developed with specific primers and probes designed by analyzing and comparing the psbA-trnH gene sequences of Picrorhiza kurroa and its similar species.A total of 29 DNA samples were tested by this method.The results showed that the real-time PCR method established in this study enabled rapid,accurate and sensitive identification of Picrorhiza kurroa.

关 键 词：库洛胡黄连 西藏胡黄连 实时荧光PCR 快速鉴别 

分 类 号：R28[医药卫生—中药学]