藏药蕨麻化学成分研究及指纹图谱结合含量测定综合评价其质量  

Study on chemical composition and evaluation of the quality of Potentilla anserina L.through fingerprint combined with content determination

作　　者：陈国飞 刘水娟 陈君 许甜 王智森 Chen Guofei;Liu Shuijuan;Chen Jun;Xu Tian;Wang Zhisen(Tibetan Medicine Quality Standard Research Office of Shijiazhuang Zangnuo Pharmaceutical Co.,Ltd.,Shijiazhuang 050035,China;Tibetan Medicine Quality Standard Research Office of Tibetan Medicine Quality and Technology Innovation Center of Hebei Province,Shijiazhuang 050035,China;Research Office of Secondary Development of Traditional Tibetan Medicine Preparations of Hebei National Pharmaceutical Preparation Industry Technology Research Institute,Shijiazhuang 050035,China)

机构地区：[1]石家庄藏诺药业股份有限公司藏药质量标准研究室,石家庄050035 [2]河北省藏药质量技术创新中心藏药质量标准研究室,石家庄050035 [3]河北省民族药制剂产业技术研究院传统藏药制剂二次开发研究室,石家庄050035

出　　处：《国际中医中药杂志》2025年第1期82-87,共6页International Journal of Traditional Chinese Medicine

基　　金：河北省创新能力提升计划项目(22567641H);西藏自治区科技计划中央引导地方项目(XZ202101YD0006C);中央引导地方科技发展资金项目(236Z2502G)。

摘　　要：目的基于超高效液相色谱-四级杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-MS/MS)分析藏药材蕨麻化学成分,结合UPLC建立其指纹图谱和含量检测方法,为其药材质量评价提供依据。方法采用智能UPLC-Q-Exactive-MS/MS结合文献及PubChem数据库辨识蕨麻药材化学成分;HPLC法建立12批蕨麻药材指纹图谱;化学模式分析识别差异标志物;定量分析质量标志物含量。结果从蕨麻中鉴定出14个化学成分,其中有黄酮类10个、油脂类3个、原花青素类1个;从蕨麻指纹图谱中确定了15个共有峰,指认出7个化学成分;化学模式识别出8个差异标志物;含量测定结果表明,不同批次蕨麻样品儿茶素、刺梨苷、野蔷薇苷、委陵菜酸、坡模酸、熊果酸含量分别为0.028~0.080 mg/g、0.144~0.361 mg/g、0.323~0.630 mg/g、0.233~0.645 mg/g、0.214~0.384 mg/g、0.115~0.203 mg/g。结论本研究可为蕨麻化学成分研究和药效物质基础研究提供参考依据,为客观评价蕨麻药材质量和其质量控制提升提供科学依据。Objective To analyze the chemical components of the Tibetan medicinal material Potentilla anserina L.based on UPLC-Q-EXACTIVE-MS/MS;To establish its fingerprint and content determination method combined with UPLC;To provide the basis for its quality evaluation.Methods Intelligent UPLC-Q-Exactive-MS/MS combined with literature and PubChem database was used to identify chemical components of Potentilla anserina L.HPLC method was used to establish fingerprints of 12 batches of Potentilla anserina L.Chemical pattern analysis was performed to identify markers of difference.The content of quality markers was quantitatively analyzed.Results 14 chemical components were identified from Potentilla anserina L,including 10 flavonoids, 3 oils and 1 proanthocyanidins;15 common peaks and 7 chemical components wereidentified from the fingerprints of Potentilla anserina L.;chemical patterns identified 8 differencemarkers;the content determination results showed that the contents of catechin, Kaji-ichigoside F1,rosamultin, tormentic acid , pomolic acid and ursolic acid in different batches were among0.028-0.080 mg/g, 0.144-0.361 mg/g, 0.323-0.630 mg/g, 0.233-0.645 mg/g, 0.214-0.384 mg/g,0.115-0.203 mg/g, respectively. Conclusion This study can provide a reference for the study ofchemical constituents and pharmacodynamic substances of Potentilla anserina L., and provide ascientific basis for the objective evaluation of the quality of Potentilla anserina L. and theimprovement of its quality control.

关 键 词：含量测定(中药) 蕨麻 UPLC-Q-Exactive-MS/MS 指纹图谱 

分 类 号：R29[医药卫生—民族医学]