电针对颅脑损伤大鼠行走功能及脑水肿相关蛋白基质金属蛋白酶-9、基质金属蛋白酶组织抑制剂-1表达影响  

Impacts of Electroacupuncture on Walking Function and Protein Expressions of Brain Edema-related Matrix Metalloproteinase 9 and Tissue Inhibitor of Matrix Metalloproteinase 1 in Rats with Traumatic Brain Injury

作　　者：汤阳阳 吴涛[1] 韦晓梅 段荣博 王瑞辉[1] TANG Yangyang;WU Tao;WEI Xiaomei;DUAN Rongbo;WANG Ruihui(Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi,China)

机构地区：[1]陕西中医药大学,陕西咸阳712046

出　　处：《辽宁中医药大学学报》2025年第3期65-72,共8页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81904310);陕西省自然科学基金青年项目(2021JQ-729)。

摘　　要：目的观察电针对创伤性颅脑损伤(traumatic brain injury,TBI)大鼠行走功能、脑组织病理结构、脑水肿相关蛋白基质金属蛋白酶-9(matrix metalloproteinase 9,MMP-9)、基质金属蛋白酶组织抑制剂-1(tissue inhibitor of matrix metalloproteinase 1,TIMP-1)表达的影响,探索电针治疗TBI可能的作用机制。方法选取SD雄性大鼠140只,随机分5组,假手术组、空白组、模型组、电针1组和电针2组,根据干预时间将模型组和电针1组分为5个亚组(干预24 h、3 d、7 d、10 d、14 d),电针2组分为2个亚组(干预10 d、14 d),10只/组。除空白组和假手术组外,用电子颅脑损伤仪(electric Cortical Contusion Impactor,eCCI)制备TBI大鼠模型;假手术组仅制造骨窗,不撞击。电针1组于造模4 h后行电针治疗,电针2组于第7天开始干预,取“百会”“关元”“足三里”等腧穴,疏密波,频率2 Hz,1次/d,15 min/次,第14天结束。分别于造模前及干预24 h、7 d、14 d通过行走功能试验评价TBI大鼠的运动功能。干预各时间点分别取材,HE染色观察脑组织形态结构;免疫组织化学、免疫印迹试验(Western blot)、实时荧光定量PCR、酶联免疫吸附试验(ELISA)观测脑组织MMP-9、TIMP-1表达水平。结果相较于空白组和假手术组,干预24 h、7 d、14 d,电针1组、电针2组与模型组行走功能评分均有所升高(P<0.01),但电针1组始终低于模型组(P<0.01);与电针1组比较,干预14 d电针2组行走功能评分较高(P<0.05)。干预24 h、3 d、7 d,与空白组和假手术组比较,其他各组均可见细胞排列紊乱,核裂解、固缩,组织空泡样改变,电针1组较模型组明显改善;干预10 d、14 d,模型组及电针1组、电针2组均可见正常神经元,且电针1组修复更佳。与假手术组比较,模型组大鼠损伤区域MMP-9表达升高而电针1组、电针2组降低(P<0.05),模型组大鼠TIMP-1表达降低而电针1组、电针2组升高(P<0.01)。与同时间点模型组比较,电针1组大�Objective In traumatic brain injury(TBI)rats,to survey the effects of electroacupuncture on walking function,brain histopathological structure,the expression of matrix metalloproteinase 9(MMP 9)and tissue inhibitor of matrix metalloproteinase 1(TIMP 1)associated with cerebral edema protein in rats with TBI,and investigating in the treatment of TBI the probable mechanism of action of electroacupuncture treatment.Methods 140 SD rats,randomized as 5 groups,sham-operated group,blank group,model group,electroacupuncture group 1 and electroacupuncture group 2.There were 10 rats in the blank group and 10 rats in sham operation group.The model group and electroacupuncture group 1 were classified into 5 subgroups(24 h,3 d,7 d,10 d and 14 d,10 rats/subgroup),and the electroacupuncture group 2 was classified into 2 subgroups(10 d and 14 d,10 rats/subgroup)in accordance with the intervention time.The TBI rat model was prepared using an electronic Cortical Contusion Impactor(eCCI);the sham-operated group was only exposed to the skull and drilled without striking.Electroacupuncture was performed in electoracupuncture group 1 at 4 h after modelling and in electoracupuncture group 2 at 7 d after modelling,and“Baihui(GV20)”“Guanyuan(RN4)”“Quchi(LI11)”“Hegu(LI4)”“Zusanli(ST36)”and“Yongquan(KI11)”were taken,sparse and dense wave,frequency 2 Hz,once a day,15 min each time.The walking function test was used to evaluate the motor function of TBI rats before modelling,24 h,7 d and 14 d,respectively.Brain tissue sampling was performed at each time point of intervention.By HE staining method,inspecting pathological morphological changes of brain tissue;to test the protein concentration of MMP-9 and TIMP-1 in brain tissue,accessing immunohistochemistry,Western blot,fluorescence quantitative PCR,ELISA.Results Walking function scores were elevated in both electroacupuncture and model groups after modelling when compared with the blank and sham-operated groups(P<0.01),but the electroacupuncture group 1 were lower than

关 键 词：颅脑损伤 电针 行走功能 脑水肿 基质金属蛋白酶-9 基质金属蛋白酶组织抑制剂-1 

分 类 号：R245.97[医药卫生—针灸推拿学]