大鼠切口疝腹膜前间隙补片修补术后血清中VEGF和Ang-1的表达情况  

Expression of VEGF and Ang-1 in serum after incisional hernia preperitoneal gap patch repair in rats

作　　者：朱佳琳 方向[1] 贵诗雨 黄丹 周小雨[1] 郭文恺 Zhu Jialin;Fang Xiang;Gui Shiyu;Huang Dan;Zhou Xiaoyu;Guo Wenkai(Department of Hernia Surgery,Deyang People's Hospital,Deyang 618000,Sichuan Province,China)

机构地区：[1]德阳市人民医院疝外科,四川省618000

出　　处：《中华疝和腹壁外科杂志(电子版)》2024年第6期703-707,共5页Chinese Journal of Hernia and Abdominal Wall Surgery(Electronic Edition)

基　　金：四川省科技计划项目基金(2019YJ0700)。

摘　　要：目的研究大鼠切口疝腹膜前间隙补片修补术后不同时间血清中血管内皮生长因子(VEGF)和血管生成素-1(Ang-1)的变化情况,探索补片与组织的融合速度。方法选用120只SD大鼠,随机分为对照组,切口疝模型组,无疝植入补片组术后1、4、8、12周组,有疝补片修补组术后1、4、8、12周组,每组大鼠12只。将聚丙烯补片植入无疝植入补片组和有疝补片修补组大鼠的腹膜前间隙。对照组取正常大鼠的血液,切口疝模型组取造模术后2周的血液,无疝植入补片组和有疝补片修补组取植入补片术后1、4、8、12周的血液,测量血清中VEGF、Ang-1含量。结果与对照组相比,切口疝模型组血清中VEGF及Ang-1浓度均明显升高(P<0.05),无疝植入补片组术后1周组VEGF及Ang-1浓度均升高(P<0.05),术后4、8、12周组VEGF浓度差异无统计学意义(P>0.05),而术后4、8周组Ang-1浓度有所升高(P<0.05),术后12周组无统计学意义(P>0.05);与切口疝模型组相比,有疝补片修补组术后1、4、8周组的VEGF及Ang-1浓度差异无统计学意义(P>0.05),术后12周组VEGF浓度降低(P<0.05),Ang-1浓度无明显差异(P>0.05);与无疝植入补片组各个时间段对比,有疝补片修补组术后12周组Ang-1水平升高(P<0.05),其余各时间段VEGF及Ang-1浓度均无明显差异(P>0.05)。结论大鼠腹外疝腹膜前间隙补片修补术后,手术创伤引起血清中VEGF及Ang-1显著增高,而补片异物的刺激并未引起血清中VEGF及Ang-1额外的显著增高,说明补片与组织的融合可能是一个缓慢的过程,提示手术后短期内应避免剧烈活动导致还没有与组织较好融合的补片移位。Objective To detect the changes of vascular endothelial growth factor(VEGF)and angiopoietin-1(Ang-1)in serum at different times after preperitoneal space mesh repair for incisional hernia in rats,and explore the fusion rate of mesh and tissue.Methods A total of 120 SD rats were randomly divided into control group(n=12),incisional hernia model group(n=12),hernia-free mesh group 1,4,8,12 weeks after surgery(n=12 in each group),and 1,4,8 and 12 weeks after hernia mesh repair(n=12 in each group).Implant polypropylene mesh into the preperitoneal space of rats in the hernia-free implantation mesh group and the hernia mesh repair group.The control group took the blood of normal rats,the incision hernia model group took the blood 2 weeks after modeling,and the blood of 1,4,8 and 12 weeks after the implantation mesh was taken from the hernia implant-free mesh group and the hernia mesh repair group,and the VEGF and Ang-1 contents in serum were measured.Results Compared with the control group,the serum concentrations of VEGF and Ang-1 in the incisional hernia model group increased significantly(P<0.05),the concentrations of VEGF and Ang-1 increased(P<0.05)in the group without hernia implantation mesh group 1 week after surgery,and there was no significant difference in VEGF concentration in the 4,8 and 12 weeks after surgery(P>0.05),while the concentration of Ang-1 increased in the 4 and 8 weeks postoperative group(P<0.05),and there was no significant difference in the group 12 weeks after surgery(P>0.05);Compared with the incision hernia model group,there was no significant difference in VEGF and Ang-1 concentrations in the hernia mesh repair group at 1,4 and 8 weeks after surgery(P>0.05),and the VEGF concentration decreased(P<0.05)and Ang-1 concentration(P>0.05)in the hernia mesh repair group.Compared with the hernia implantation mesh group,the level of Ang-1 increased(P<0.05)in the hernia mesh repair group 12 weeks after surgery,and there was no significant difference in VEGF and Ang-1 concentrations in the remaining per

关 键 词：切口疝 腹膜前间隙 补片 疝修补术 血管内皮生长因子 血管生成素-1 

分 类 号：R73[医药卫生—肿瘤]