Tol-Pal系统tolQ基因对禽致病性大肠杆菌生物学特性及毒力的影响  

Tol-Pal system tolQ gene affects the biological characteristics and virulence of avian pathogenic Escherichia coli

作　　者：李双阳 张贝贝 胡剑刚 王欣宇 祁晶晶[2] 田明星 鲍衍清[2] 石玉祥 王少辉 LI Shuangyang;ZHANG Beibei;HU Jiangang;WANG Xinyu;QI Jingjing;TIAN Mingxing;BAO Yanqing;SHI Yuxiang;WANG Shaohui(School of Life Science and Food Engineering,Hebei University of Engineering,Handan 056001,China;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China)

机构地区：[1]河北工程大学生命科学与食品工程学院,河北邯郸056001 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国兽医科学》2025年第1期11-17,共7页Chinese Veterinary Science

基　　金：国家自然科学基金项目(32172856);河北省三期现代农业产业技术体系蛋禽创新团队资助项目(HBCT2024260205);上海市自然科学基金项目(22ZR1476100,23ZR1476600);中国农业科学院科技创新工程项目(CAAS-ASTIP-2021-SHVRI-07)。

摘　　要：本研究利用Red同源重组系统和互补质粒构建禽致病性大肠杆菌(avian pathogenic Escherichia coli,APEC)菌株APCE50的tolQ基因缺失株ΔtolQ和互补株CΔtolQ,以探究tolQ基因对APEC生物学特性以及对细菌致病性的影响。对APCE50、ΔtolQ和CΔtolQ的生长曲线、运动能力、形态特征、生物被膜形成能力、酸碱耐受性、抗血清杀菌、细胞黏附侵袭能力及毒力进行了比较分析。结果显示,ΔtolQ与APCE50、CΔtolQ的生长速度无明显差异,但ΔtolQ的运动能力下降并影响细菌形态。缺失tolQ基因导致APEC生物被膜形成能力、酸碱耐受性及抗血清杀菌能力显著降低。细胞及动物感染试验结果显示,tolQ基因缺失显著降低了APEC的黏附侵袭能力和毒力。综上表明,tolQ基因在APEC运动能力、生物被膜形成、耐受性及致病性方面发挥作用,这为阐明APEC的环境适应性、生存能力及致病机制提供了参考。This study focused on examining the role of the tol Q gene in the biological characteristics and pathogenicity of avian pathogenic Escherichia coli(APEC).Using the Red homologous recombination system and complementation plasmid,tol Q gene mutant and complemented strains of APEC were constructed.Then,we compared the the growth patterns,motility,morphology,biofilm formation capacity,survival in acidic and alkaline environments,cell adhesion and invasion capabilities,and pathogenicity of APCE50,Δtol Q,and CΔtol Q strains.The results indicated that the mutant strain exhibited decreased motility and altered bacterial morphology.The absence of the tol Q gene led to significant decreased biofilm formation capacity,reduced survival rate in acidic and alkaline conditions,and diminished serum bactericidal resistance.Cell and animal infection assays revealed that the tol Q mutant strain exhibited significantly diminished capacity to adhere to and invade cells,as well as attenuated virulence in animal.In summary,the tol Q gene plays a significant role in APEC motility,biofilm formation,tolerance,and pathogenicity.These findings provide insights into the environmental adaptation,survival,and pathogenic mechanisms of APEC.The study contributes to our understanding of the environmental adaptability,adhesion and invasion abilities,and virulence mechanisms of APEC.

关 键 词：禽致病性大肠杆菌 tolQ 生物学特性 毒力 

分 类 号：S852.612[农业科学—基础兽医学]