艾灸对糖尿病认知功能减退大鼠中枢胰岛素抵抗相关蛋白的影响  

Effect of moxibustion on central insulin resistance related proteins in diabetic rats with cognitive decline

作　　者：叶敏 袁爱红[2] 张乐乐 解鸿宇 宋蝴蝶 樊吟秋 杨骏[2] YE Min;YUAN Aihong;ZHANG Lele;XIE Hongyu;SONG Hudie;FAN Yinqiu;YANG Jun(First School of Clinical Medicine,Anhui University of CM,Hefei 230031,China;Acupuncture-Moxibustion and Rehabilitation Department,First Affiliated Hospital of Anhui University of CM,Hefei 230031)

机构地区：[1]安徽中医药大学第一临床医学院,合肥230031 [2]安徽中医药大学第一附属医院针灸康复科,合肥230031

出　　处：《中国针灸》2025年第2期185-192,共8页Chinese Acupuncture & Moxibustion

基　　金：安徽省自然科学基金项目:2108085MH308;第九批安徽省特支计划人才项目:皖组办字[2023]35号;袁爱红安徽省名中医工作室项目:皖中医药发展秘[2023]23号;杨骏全国名中医传承工作室项目:皖中医药发展秘[2022]19号。

摘　　要：目的:观察艾灸对糖尿病认知功能减退大鼠中枢胰岛素抵抗相关蛋白的影响,探讨艾灸改善认知功能的可能机制。方法:使用高脂饲料联合链脲佐菌素(STZ)腹腔注射制备糖尿病认知功能减退大鼠模型,随机取20只造模成功的大鼠分为模型组和艾灸组,每组10只,另设10只大鼠为空白组。艾灸组艾灸“百会”“神庭”及“大椎”,3穴同时悬灸,每次20 min,每周1次,每周6次,连续4周。采用血糖仪检测大鼠随机血糖水平,水迷宫试验检测大鼠学习记忆能力,HE染色观察大鼠海马组织神经元形态,实时荧光定量PCR法检测大鼠海马组织胰岛素受体底物1(IRS1)、磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)mRNA表达,Western blot法检测大鼠海马组织IRS1、PI3K、AKT、磷酸化胰岛素受体底物1(p-IRS1)、磷酸化磷脂酰肌醇3-激酶(p-PI3K)、磷酸化蛋白激酶B(p-AKT)蛋白表达并计算p-IRS1/IRS1、p-PI3K/PI3K、p-AKT/AKT的比值,免疫荧光法观察大鼠海马组织p-IRS1、p-PI3K、p-AKT荧光强度。结果:与空白组比较,模型组大鼠随机血糖水平升高(P<0.001),逃避潜伏期延长(P<0.001),海马区病理损伤严重,海马组织IRS1、PI3K、AKT mRNA表达降低(P<0.001),p-IRS1/IRS1、p-PI3K/PI3K、p-AKT/AKT比值降低(P<0.001),海马组织p-IRS1、p-PI3K、p-AKT荧光强度降低(P<0.001);与模型组比较,艾灸组大鼠随机血糖水平降低(P<0.05),逃避潜伏期缩短(P<0.01),海马区病理损伤改善,海马组织IRS1、PI3K、AKTmRNA表达升高(P<0.01),p-IRS1/IRS1、p-PI3K/PI3K、p-AKT/AKT比值升高(P<0.01,P<0.05),海马组织p-IRS1、p-PI3K、p-AKT荧光强度升高(P<0.01,P<0.05)。结论:艾灸可改善糖尿病认知功能减退大鼠的学习记忆能力,可能与调节海马区IRS1、PI3K、AKT蛋白表达及其磷酸化水平,激活并促进胰岛素信号转导,改善中枢胰岛素抵抗有关。Objective To investigate the effect of moxibustion on central insulin resistance related proteins of the rats suffering from diabetic cognitive decline,and analyze the underlying mechanism of moxibustion for cognition improvement.Methods Using the intraperitoneal injection of STZ combined with a high-fat diet,the rat model of diabetic cognitive decline were prepared.Twenty successfully-modeled rats were assigned randomly into a model group and a moxibustion group,10 rats in each one.Besides,a blank group was set up with 10 rats collected.In the moxibustion group,suspending moxibustion was applied to"Baihui"(GV20),"Shenting"(GV24)and"Dazhui"(GV14)at the same time,20 min in each intervention,once a day,and 6 interventions were delivered weekly and the duration of treatment was consecutive 4 weeks.The random blood glucose was measured using glucometer,and the learning-memory ability was detected by water maze test.HE staining was used to observe the morphology of neurons in the hippocampal tissue,real-time PCR assay was to detect mRNA expression of insulin receptor substrate 1(IRS1),phosphatidylinositol 3-kinase(PI3K)and protein kinase B(AKT)in the hippocampal tissue.The Western blot method was employed to detect the protein expression of IRS1,PI3K,AKT,phosphorylated IRS1(p-IRS1),phosphorylated PI3K(p-PI3K)and phosphorylated AKT(p-AKT)in the hippocampal tissue,and the ratio of p-IRS1/IRS1,p-PI3K/PI3K and p-AKT/AKT was calculated separately.The immunofluorescence intensity of p-IRS1,p-PI3K,and p-AKT was measured using immunofluorescence.Results Compared with the blank group,the rats of the model group exhibited higher random blood glucose(P<0.001),longer escape latency(P<0.001),severe pathological damage in the hippocampus,lower mRNA expression of IRS1,PI3K,and AKT(P<0.001),reduced ratio of p-IRS1/IRS1,p-PI3K/PI3K and p-AKT/AKT(P<0.001),and declined immunofluorescence intensity of p-IRS1,p-PI3K,and p-AKT in the hippocampal tissue(P<0.001).In comparison with the model group,for the rats of the moxibustion group,the ra

关 键 词：糖尿病认知功能减退 艾灸 中枢胰岛素抵抗 PI3K/AKT 

分 类 号：R245.81[医药卫生—针灸推拿学]