电针对脑缺血再灌注损伤学习记忆障碍大鼠线粒体自噬及Sirt1/FOXO3/PINK1/Parkin通路的影响  

Effects of electroacupuncture on mitochondrial autophagy and Sirt1/FOXO3/PINK1/Parkin pathway in rats with learning-memory impairment after cerebral ischemia reperfusion injury

作　　者：苏凯奇 吕转 张铭[1] 陈露露 刘昊 高静 冯晓东[1,2] SU Kaiqi;LV Zhuan;ZHANG Ming;CHEN Lulu;LIU Hao;GAO Jing;FENG Xiaodong(Rehabilitation Center,First Affiliated Hospital of Henan University of CM,Zhengzhou 450000,China;College of Rehabilitation Medicine,Henan University of CM,Zhengzhou 450046)

机构地区：[1]河南中医药大学第一附属医院康复中心,郑州450000 [2]河南中医药大学康复医学院,郑州450046

出　　处：《中国针灸》2025年第2期193-199,共7页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金面上项目:82174473;国家自然科学基金青年基金项目:82305364;河南省科技研发计划联合基金(优势学科培育类)项目:232301420082;河南省卫健委国家中医药传承创新中心科研专项:2023ZXZX1131。

摘　　要：目的:观察电针“神庭”“百会”对脑缺血再灌注损伤后学习记忆障碍大鼠海马神经元线粒体自噬及沉默信息调节因子1(Sirt1)/叉形头转录因子O3(FOXO3)/PTEN诱导激酶1(PINK1)/Parkin通路的影响。方法:将35只雄性SD大鼠随机分为假手术组(9只)和造模组(26只),造模组采用大脑中动脉阻塞法构建大脑中动脉栓塞/再灌注(MCAO/R)模型,将造模成功的18只大鼠随机分为模型组和电针组,各9只。电针组予电针“神庭”“百会”干预,每次30 min,每天1次,连续14 d。于造模后及干预第7、14天观察大鼠神经功能缺损评分;Morris水迷宫实验检测大鼠学习记忆能力;尼氏染色法观察大鼠海马CA1区神经元形态;透射电镜观察大鼠海马CA1区神经元线粒体超微结构;Western blot法检测大鼠海马Beclin-1、微管相关蛋白轻链3B(LC3B)、P62、Sirt1、FOXO3、PINK1、Parkin蛋白表达。结果:造模后,模型组和电针组大鼠神经功能缺损评分高于假手术组(P<0.001);干预第7、14天,模型组大鼠神经功能缺损评分高于假手术组(P<0.001),电针组大鼠神经功能缺损评分低于模型组(P<0.05,P<0.01)。造模后,模型组和电针组大鼠逃避潜伏期较假手术组延长(P<0.001);干预第9~13天,模型组大鼠逃避潜伏期较假手术组延长(P<0.001);电针组大鼠逃避潜伏期较模型组缩短(P<0.05,P<0.01,P<0.001)。模型组大鼠穿越平台次数较假手术组减少(P<0.001);电针组大鼠穿越平台次数较模型组增加(P<0.05)。模型组大鼠海马CA1区神经元数目减少,排列稀疏,核固缩,胞浆深染,尼氏小体减少;线粒体形态肿胀,膜结构不完整,自噬溶酶体形成。与模型组比较,电针组大鼠海马CA1区神经元数量增多,异常形态细胞减少,尼氏小体数量增多;线粒体形态较完整,自噬溶酶体数量增多。与假手术组比较,模型组大鼠海马Beclin-1、FOXO3、PINK1、Parkin蛋白表达及LC3Ⅱ/Ⅰ比值升高(P<0.01,P<0.001),P62蛋白表达Objective To observe the effects of electroacupuncture(EA)at"Shenting"(GV24)and"Baihui"(GV20)on mitochondrial autophagy in hippocampal neurons and silent information regulator sirtuin 1(Sirt1)/forkhead box O3(FOXO3)/PTEN-inducible kinase 1(PINK1)/Parkin pathway in rats with learning-memory impairment after cerebral ischemia reperfusion injury.Methods A total of 35 male SD rats were randomly divided into a sham operation group(9 rats)and a modeling group(26 rats).In the modeling group,middle cerebral artery occlusion method was used to establish the middle cerebral artery ischemia-reperfusion(MCAO/R)model,and 18 rats of successful modeling were randomly divided into a model group and an EA group,9 rats in each one.EA was applied at"Shenting"(GV24)and"Baihui"(GV20)in the EA group,30 min a time,once a day for 14 days.After modeling and on 7th and 14th days of intervention,neurologic deficit score was observed;the learning-memory ability was detected by Morris water maze test;the morphology of neurons in CA1 area of hippocampus was detected by Nissl staining;the mitochondrial morphology was observed by transmission electron microscopy;the protein expression of Beclin-1,microtubule-associated protein 1 light chain 3B(LC3B),P62,Sitrt1,FOXO3,PINK1 and Parkin was detected by Western blot.Results After modeling,the neurologic deficit scores in the model group and the EA group were higher than that in the sham operation group(P<0.001);on 7th and 14th days of intervention,the neurologic deficit scores in the model group were higher than those in the sham operation group(P<0.001),the neurologic deficit scores in the EA group were lower than those in the model group(P<0.05,P<0.01).After modeling,the escape latency in the model group and the EA group was prolonged compared with that in the sham operation group(P<0.001);on 9th-13th days of intervention,the escape latency in the model group was prolonged compared with that in the sham operation group(P<0.001),the escape latency in the EA group was shortened compared with that in

关 键 词：脑缺血再灌注损伤 学习记忆障碍 电针 神庭 百会 线粒体自噬 

分 类 号：R245[医药卫生—针灸推拿学]