促凋亡蛋白Bid/C1q对酮病奶牛脂肪细胞凋亡及炎症的影响  

Effect of pro-apoptotic protein Bid/C1q on adipocyte apoptosis and inflammation in ketosis cows

作　　者：李晴 孙晗冰 向念 许秋实[1] 徐闯[1] LI Qing;SUN Hanbing;XIANG Nian;XU Qiushi;XU Chuang(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163000,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163000

出　　处：《中国兽医学报》2024年第11期2477-2482,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32102743,32125038)

摘　　要：奶牛酮病经常伴随着脂肪组织中凋亡及炎症的反应。为了研究促凋亡蛋白Bid对酮病奶牛脂肪细胞凋亡的影响,本研究通过TUNEL染色技术对脂肪组织进行染色,观察酮病奶牛脂肪组织凋亡变化。体内试验中,通过Western blot检测健康奶牛及酮病奶牛脂肪组织中凋亡相关因子Bid、Bax、C-Caspase-3、Bcl-2及炎症标志因子C1q、IL-1β、IL-10和IL-6的蛋白表达。体外试验中,本研究通过体外培养原代牛脂肪细胞抑制Bid,并添加异丙肾上腺素(ISO)构建脂肪细胞脂解模型,采用Western blot技术检测脂肪细胞中凋亡相关分子及炎症相关分子的蛋白表达水平。TUNEL染色结果显示:与健康奶牛相比,酮病奶牛组脂肪组织明显发生了凋亡,酮病奶牛脂肪组织中的促凋亡相关因子Bid、Bax和C-Caspase-3及促炎症标志因子TNF-α、IL-1β、和IL-6的蛋白表达均显著升高,补体C1q、抗凋亡因子Bcl-2及抗炎症因子IL-10显著降低。体外结果显示:与对照组相比,ISO组处理的脂肪细胞凋亡和炎症相关因子蛋白表达水平明显升高;而添加Bid抑制剂处理组脂肪细胞凋亡和炎症相关因子蛋白表达水平明显降低。上述结果显示,抑制Bid可以缓解ISO对脂肪细胞的凋亡及炎症反应。进一步明确了Bid/C1q对酮病奶牛脂肪组织及细胞凋亡及炎症的调控作用。Ketosis in dairy cows is often accompanied by apoptotic and inflammatory responses in adipose tissue.In order to investigate the effect of pro-apoptotic protein Bid on adipocyte apoptosis in cows with ketosis,the adipose tissue was stained by TUNEL staining technique in this study to observe the apoptotic changes in adipose tissue of cows with ketosis.In the in vivo test,protein expression of apoptosis-related factors Bid,Bax,C-Caspase-3,Bcl-2 and inflammation marker factors C1q,IL-1β,IL-10 and IL-6 in adipose tissues of healthy cows and ketosis cows were detected by Western blot.In the in vitro assay,the adipocyte lipolysis model was constructed by culturing primary bovine adipocytes in vitro to inhibit Bid and adding isoproterenol(ISO),and the protein expression levels of apoptosis-related molecules and inflammation-related molecules in adipocytes were detected by Western blot technique.The results of TUNEL staining showed that the protein expression of pro-apoptotic factors Bid,Bax and C-Caspase-3,and pro-inflammatory markers TNF-α,IL-1β,and IL-6 were significantly higher,and the protein expression of complement C1q,anti-apoptotic factor Bcl-2,and anti-inflammatory factor IL-10 were significantly lower in adipose tissues of ketosis cows compared with that of healthy cows.The in vitro results showed that the protein expression levels of apoptosis and inflammation-related factors in adipocytes treated with the ISO group were significantly higher compared with those in the control group,while the protein expression levels of apoptosis and inflammation-related factors in adipocytes treated with the addition of the Bid inhibitor group were significantly lower.The above results showed that inhibition of Bid could alleviate the apoptotic and inflammatory responses of ISO on adipocytes.This will further clarify the role of Bid/C1q in the regulation of adipose tissue and cell apoptosis and inflammation in ketosis cows.

关 键 词：奶牛酮病 脂肪细胞 BID 凋亡 炎症 

分 类 号：S856.5[农业科学—临床兽医学]