硒代蛋氨酸对AFB1致家兔胸腺损伤的保护作用  

Protective effect of selenomethionine on thymus damage in rabbits induced by AFB1

作　　者：孔德婧 王诗诗 张智凯 刘玉梅[1] 刘世洋 张自强[1] KONG Dejing;WANG Shishi;ZHANG Zhikai;LIU Yumei;LIU Shiyang;ZHANG Ziqiang(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang,Henan 471000,China)

机构地区：[1]河南科技大学动物科技学院,河南洛阳471000

出　　处：《中国兽医学报》2024年第11期2470-2476,共7页Chinese Journal of Veterinary Science

基　　金：河南省重点研发与推广专项资助项目(202102110093);校企横向合作项目资助项目(横20210018)

摘　　要：黄曲霉毒素B1(aflatoxin,AFBl)是黄曲霉和寄生曲霉菌产生的次级代谢产物,具有很强的免疫毒性和致癌性,严重危害人类和家畜健康。本研究旨在探讨硒代蛋氨酸(selenomethionine,SeMet)缓解AFB1诱导的胸腺损伤的作用。50只35日龄家兔随机分为5组,每组10只,分别为对照组、AFB1组、AFB1+0.2Se组(0.2 mg/kg SeMet)、AFB1+0.4Se组(0.4 mg/kg SeMet)及AFB1+0.6Se组(0.6 mg/kg SeMet),试验期为21 d。第17天时,AFB1组和各硒代蛋氨酸组每只兔每天灌胃AFB1(0.3 mg/kg),连续5 d。取家兔胸腺组织进行HE、TUNEL和PCNA检测。并采用ELISA检测胸腺组织炎性因子表达量,通过GSH-Px、T-AOC和MDA试剂盒检测胸腺组织氧化应激指标。结果表明,AFB1暴露导致家兔胸腺组织中MDA水平极显著升高116.04%,抗氧化酶GSH-Px和T-AOC活性分别显著降低29.20%和52.17%。此外,AFB1诱导胸腺发生炎症反应,与对照组相比,促进TNF-α分泌增加124.71%、IL-6增加174.72%和IL-1β增加62.38%。硒代蛋氨酸预处理显著改善了家兔的胸腺病理变化,减轻了其氧化应激和炎症反应。本研究证实了硒代蛋氨酸可缓解AFB1诱导的胸腺损伤,提高家兔的免疫性能。Aflatoxin B1(AFB1)is a secondary metabolite produced by Aspergillus aflatoxin and Aspergillus parasiticus.It has strong immunotoxicity and carcinogenicity and seriously harms the health of humans and livestock.Therefore,this study aims to explore the effect of selenomethionine(SeMet)in alleviating AFB1-induced thymic damage.Fifty 35-day-old rabbits were randomly divided into 5 groups,with 10 rabbits in each group.They are the control group,AFB1 group,AFB1+0.2Se group(0.2 mg/kg SeMet),AFB1+0.4Se group(0.4 mg/kg SeMet)and AFB1+0.6Se group(0.6 mg/kg SeMet).The test period was 21 d.On the 17th day,each rabbit in the AFB1 group and each SeMet group was gavaged with AFB1(0.3 mg/kg)every day for 5 consecutive days.Rabbit thymus tissue was taken for HE,TUNEL and PCNA detection.ELISA was used to detect the expression of inflammatory factors in thymus tissue,and GSH-Px,T-AOC and MDA kits were used to detect oxidative stress indicators in thymus tissue.The results showed that AFB1 exposure caused a very significant increase in MDA levels in rabbit thymus tissue by 116.04%,and a significant decrease in the activities of antioxidant enzymes GSH-Px and T-AOC by 29.20%and 52.17%respectively.In addition,AFB1 induced an inflammatory response in the thymus,promoting TNF-αsecretion to increase by 124.71%,IL-6 by 174.72%,and IL-1βby 62.38%compared with the control group.SeMet pretreatment significantly improved the pathological changes of the thymus in rabbits and reduced its oxidative stress and inflammatory response.Therefore,we confirmed that SeMet alleviates AFB1-induced thymic damage and improves the immune performance of rabbits.

关 键 词：硒代蛋氨酸 AFB1 胸腺 免疫 家兔 

分 类 号：S816.7[农业科学—饲料科学]