酮病奶牛CD4^(+)T细胞中丝氨酸代谢变化与炎症变化  

Changes in serine metabolism and inflammation in CD4^(+)T cells of cows with ketosis

作　　者：王晶晶 李铭 温佳楠 王爽[3] 徐闯 杨威[3] 张冰冰[1] WANG Jingjing;LI Ming;WEN Jianan;WANG Shuang;XU Chuang;YANG Wei;ZHANG Bingbing(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;College of Veterinary Medicine,China Agricultural University,Beijing 1001912,China;College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [2]中国农业大学动物医学院,北京100191 [3]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2024年第11期2445-2451,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32172926,U20A2062)

摘　　要：为探究健康和酮病奶牛CD4^(+)T细胞中丝氨酸代谢相关基因的表达及炎症相关基因的表达。首先使用磁珠分选的方法,分离奶牛外周血中CD4^(+)T细胞。其次,使用荧光定量PCR方法检测健康、酮病奶牛CD4^(+)T细胞中丝氨酸代谢相关基因PHGDH、PAST1、PSPH、SHMT1、SHMT2、SDS、SFXN1及炎症相关基因IL-6、IFN-γ、IL-17A、FOXP3、IL-10、TGF-β的表达。与健康奶牛相比,酮病奶牛CD4^(+)T细胞中PHGDH、PAST1和PSPH的转录水平均极显著升高(P<0.01),SDS的转录水平与翻译水平均显著升高(P<0.01)。而SHMT2的转录水平显著降低(P<0.05)。SFXN1的转录水平与翻译水平均显著降低(P<0.05)。SHMT1的转录水平降低但无显著性。与健康奶牛相比,酮病奶牛CD4^(+)T细胞中IL-6、IFN-γ、IL-17A的转录水平均极显著升高(P<0.01),IL-10、TGF-β、FOXP3的转录水平均显著降低(P<0.05)。结果表明,酮病奶牛丝氨酸合成过程基因表达升高,丝氨酸分解过程相关基因表达降低,而促炎因子表达升高,抑炎因子表达降低,说明丝氨酸代谢在奶牛体内发生炎症过程中发挥重要作用。To investigate the expression of serine metabolism related genes and inflammatory related genes in CD4^(+)T cells of healthy and cows with ketosis.Firstly,CD4^(+)T cells in peripheral blood of dairy cows were isolated by magnetic bead sorting.Second,expression of serine metabolism related genes PHGDH,PAST1,PSPH,SHMT1,SHMT2,SDS,SFXN1 and inflammation related genes IL-6,IFN-γ,IL-17A,FOXP3,IL-10,TGF-βin CD4^(+)T cells in healthy and ketosis cows was detected by fluorescence quantitative PCR.The transcription levels of PHGDH,PAST1 and PSPH in CD4^(+)T cells of ketosis cows were significantly increased compared with healthy cows(P<0.01),the transcription level and translation level of SDS were significantly increased(P<0.01).The transcription levels of SHMT2 were significantly decreased(P<0.05).Transcription and translation levels of SFXN1 were significantly decreased(P<0.05).The transcription level of SHMT1 was decreased but not significantly.Compared with healthy cows,the transcription levels of IL-6,IFN-y and IL-17 A in CD4^(+)T cells of ketosis cows were significantly increased(P<0.01),the transcription levels of IL-10,TGF-βand FOXP3 were significantly decreased(P<0.05).The results showed that the expression of genes related to serine synthesis increased,the expression of genes related to serine decomposition decreased,the expression of pro-inflammatory factors increased,and the expression of anti-inflammatory factors decreased,suggesting that serine metabolism plays an important role in the inflammatory process of dairy cows.

关 键 词：CD4^(+)T细胞 丝氨酸代谢 炎症 酮病 奶牛 

分 类 号：S852.21[农业科学—基础兽医学]