NLRX1通过促进ROS分泌抑制NDV增殖  

NLRX1 inhibits NDV proliferation by promoting ROS secretion

作　　者：付永恒 李金斗 丁佳欣 丁壮 徐小洪 FU Yongheng;LI Jindou;DING Jiaxin;DING Zhuang;XU Xiaohong(College of Veterinary Medicine,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医学报》2024年第11期2324-2333,共10页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32002321);吉林省科技厅重点研发资助项目(20230202082NC);国家自然科学基金资助项目(32072860)

摘　　要：为探讨核苷酸结合寡聚化结构域受体家族成员X1(nucleotide-binding domain and leucine-rich-repeat-containing family memberX1,NLRX1)在新城疫病毒(Newcastle disease virus, NDV)复制过程中的作用,本研究检测了NDV NA-1毒株感染10日龄SPF雏鸡和HD11细胞后NLRX1的表达水平,以及病毒在NLRX1过表达或低表达时的NDV的增殖情况、HD11细胞促炎因子的表达情况和氧化应激状态。结果表明:NA-1感染可引起体内外NLRX1表达的增加;NLRX1过表达则会抑制病毒的增殖,提高细胞IL-1β、IL-6和IFN-β的表达。而NLRX1升高不会引起细胞自噬和凋亡细胞水平的变化,但是会使细胞在24 h内iNOS、Keap1、Nrf2、NQO1和HO-1的mRNA水平均升高。综上,NLRX1可能通过促进早期ROS的产生从而抑制NDV的增殖。To investigate the role of the nucleotide-binding domain and leucine-rich-repeat-containing family member X1(NLRX1)in the replication of Newcastle disease virus(NDV),the expression level of NLRX1 was examined following infection of 10-day-old specific pathogen-free(SPF)chicks and HD11 cells with the NDV NA-1 strain.Additionally,the proliferation of NDV,the expression of pro-inflammatory cytokines in HD11 cells,and the oxidative stress status were evaluated in the presence of either overexpressed or underexpressed NLRX1.The results demonstrated that NA-1 infection led to an increase in NLRX1 expression both in vivo and in vitro.Furthermore,overexpression of NLRX1 inhibited viral proliferation,enhanced the expression of cellular IL-1β,IL-6,and IFN-β,without affecting levels of autophagy or apoptotic cells.However,NLRX1 elevation resulted in elevated mRNA levels of iNOS,Keap1,Nrf2,NQO1,and HO-1 within 24 h.In conclusion,NLRX1 suppresses NDV proliferation by promoting early ROS production.

关 键 词：新城疫病毒 NLRX1 INOS 活性氧 

分 类 号：S852.65[农业科学—基础兽医学]