水蒸气蒸馏提取和超临界CO 2流体萃取艾叶挥发油抗特应性皮炎的药效成分和作用靶点的对比分析  

Comparative analysis of the active components and targets of Artemisia argyi essential oil for atopic dermatitis extracted by steam distillation and supercritical fluid extraction

作　　者：周贝 王伟雄 赵子博 郭玉英 胡裕泽 王一飞 ZHOU Bei;WANG Weixiong;ZHAO Zibo;GUO Yuying;HU Yuze;WANG Yifei(College of Pharmacy,Jinan University,Guangzhou 511436,Guangdong,China;Departmen of Cell Biology,College of Life Science and Technology,Jinan University,Guangzhou 510632,Guangdong,China;Key Laboratory of Innovative Technology Research on Natural Products and Cosmetics Raw Materials,Guangzhou 510632,Guangdong,China;Guangzhou Jinan Biomedical Research and Development Center Co.Ltd,Guangzhou 510632,Guangdong,China;Artemisia argyi Branch Center,National Engineering Research Center for Modernization of Traditional Chinese Medicine,Guangzhou 510632,Guangdong,China)

机构地区：[1]暨南大学药学院,广东广州511436 [2]暨南大学生命科学技术学院细胞生物学系,广东广州510632 [3]天然产物化妆品原料创新技术研究重点实验,广东广州510632 [4]广州暨南生物医药研究开发基地有限公司,广东广州510632 [5]国家中药现代化工程技术研究中心艾草分中心,广东广州510632

出　　处：《暨南大学学报(自然科学与医学版)》2025年第1期82-97,共16页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(82373917);广州市重点研发计划项目(202206010008)。

摘　　要：目的:基于网络药理学和细胞体外实验,对比分析水蒸气蒸馏提取和超临界CO 2流体萃取艾叶挥发油抗特应性皮炎的药效成分和作用机理,为以艾叶挥发油为基础开发抗特应性皮炎的药物提供理论依据。方法:分别使用水蒸气蒸馏法和超临界CO 2流体萃取法提取艾叶挥发油,使用气相色谱-质谱法测定其化学成分。使用网络药理学方法分析2种艾叶挥发油抗特应性皮炎的相关靶点和通路,并使用脂多糖(lipopolysaccharides,LPS)诱导的RAW264.7细胞炎症模型探究2种挥发油抗特应性皮炎的活性。结果:水蒸气蒸馏法提取挥发油(Artemisia argyi essential oil obtained by steam distillation,AEOSD)中有65种化合物,超临界CO 2流体萃取挥发油(Artemisia argyi essential oil obtained by supercritical fluid extraction,AEOSFE)中有89种化合物,有44种化合物同时存在于2种挥发油中。网络药理学分析发现,桉叶油醇、α-蛇麻烯是AEOSD抗特应性皮炎的主要潜在药效成分,丁香酚、桉叶油醇、α-蒎烯是AEOSFE抗特应性皮炎的主要潜在药效成分;AEOSD可能通过调控一氧化氮(nitric oxide,NO)的生物合成过程、白细胞介素-6(interleukin-6,IL-6)正向调节生物过程、NOD样受体信号通路等来影响特应性皮炎的进展,而AEOSFE可能通过调控NO的生物合成过程、辅助性T细胞17(T helper cell 17,Th17)的分化、PI3K-Akt信号通路及炎症介质调节TRP通道等来影响特应性皮炎的进展。体外实验结果表明,AEOSD和AEOSFE均能降低LPS诱导的RAW264.7细胞的NO过度释放,可分别减少靶点炎症因子IL-1β、IL-6、TNF和IL-1β、IL-6、PTGS2的表达。结论:AEOSD和AEOSFE在化学成分组成和抗特应性皮炎的作用靶点通路上有所不同,但对RAW264.7细胞的抗炎活性均较好。Objective:To compare and analyze the anti-atopic dermatitis active components and mechanisms of action of Artemisia argyi essential oil extracted by water distillation and supercritical CO 2 fluid extraction based on network pharmacology and in vitro cell experiments.Methods:The essential oils of Artemisia argyi were extracted by water distillation and supercritical CO 2 fluid extraction,and their chemical components were determined by gas chromatography-mass spectrometry.Network pharmacology was used to predict the related targets and pathways of the two essential oils against atopic dermatitis,and the anti-atopic dermatitis activities of the two essential oils were explored in the lipopolysaccharides(LPS)-induced RAW264.7 cell inflammation model.Results:There were 65 compounds in Artemisia argyi essential oil obtained by steam distillation(AEOSD),89 compounds in Artemisia argyi essential oil obtained by supercritical fluid extraction(AEOSFE),and 44 common compounds in the two essential oils.Network pharmacology analysis results found that eucalyptol and humulene were the main potential active ingredients of AEOSD against atopic dermatitis,eugenol,eucalyptol,and Copaene were the main potential active ingredients of AEOSFE against atopic dermatitis.AEOSD may affect the progression of atopic dermatitis by regulating the biosynthesis of nitric oxide(NO),the positive regulation of interleukin-6(IL-6)production,and the NOD-like receptor signaling pathway,while AEOSFE may affect the progression of atopic dermatitis by regulating the biosynthesis of NO,T helper cell 17(Th17)cell differentiation,PI3K-Akt signaling pathway,and inflammatory mediator regulation of TRP channels.In vitro experimental results showed that both AEOSD and AEOSFE could reduce the excessive release of NO in RAW264.7 cells induced by LPS,and reduce the expression of their target inflammatory factors IL-1β,IL-6,TNF and IL-1β,IL-6,PTGS2,respectively.Conclusion:AEOSD and AEOSFE differ in their chemical composition and target pathways against atopic

关 键 词：艾叶挥发油 特应性皮炎 网络药理学 水蒸气蒸馏 超临界CO 2流体萃取 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]