高效液相色谱法同时测定7种药食同源物质功能成分  

Simultaneous determination of functional components of 7 kinds of medicinal and food homologous substances by high performance liquid chromatography

作　　者：高惠惠 赵亚雄 韩张斌 李海恬 杨成彦 唐云 GAO Hui-Hui;ZHAO Ya-Xiong;HAN Zhang-Bin;LI Hai-Tian;YANG Cheng-Yan;TANG Yun(Jinhui Liquor Co.,Ltd.,Longnan 742308,China)

机构地区：[1]金徽酒股份有限公司,陇南742308

出　　处：《食品安全质量检测学报》2025年第5期127-133,共7页Journal of Food Safety and Quality

摘　　要：目的 建立高效液相色谱法(high performance liquid chromatography,HPLC)同时测定药食同源性物质葛根、槐花、金银花、肉苁蓉、罗汉果、人参、枳椇子提取物中功能成分的检测方法。方法 采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm, 5μm),以乙腈-0.1%磷酸为流动相梯度洗脱,流速0.6 mL/min,柱温30℃,检测波长210 nm,进样量5μL,测定葛根素、芦丁、绿原酸、松果菊苷、毛蕊花糖苷、罗汉果甜苷V、人参皂苷Re及二氢杨梅素的含量。结果 8种功能成分在各自浓度范围内线性关系良好,相关系数(r^(2))>0.999,检出限为0.02~1.88mg/L,定量限为0.08~3.62mg/L,精密度实验结果为相对标准偏差(relative standard deviation, RSD)<3%,平均加标回收率为95.49%~109.87%。结论 该方法简便快速,准确度高,可作为上述7种药食同源性物质功能成分的定性定量分析方法。Objective To establish a method for the simultaneous determination of functional components in extracts from 7 kinds of medicinal and food homologous substances:Pueraria lobata,Sophora japonica,Lonicera japonica,Cistanche deserticola,Siraitia grosvenorii,Panax ginseng and Hovenia dulcis by high performance liquid chromatography(HPLC).Methods The analysis was conducted using an Agilent Eclipse XDB-C18 column(4.6 mm×250 mm,5μm)with a mobile phase gradient elution of acetonitrile-0.1%phosphoric acid at a flow rate of 0.6 mL/min,a column temperature of 30℃,a detection wavelength of 210 nm,and an injection volume of 5μL.This method was employed to quantify the content of puerarin,rutin,chlorogenic acid,echinacoside,verbascoside,mogroside V,ginsenoside Re and dihydromyricetin.Results The results demonstrated that 8 kinds of functional components exhibited excellent linear relationships within their respective concentration ranges,with correlation coefficients(r^(2))exceeding 0.999.The limits of detection ranged from 0.02 to 1.88 mg/L,and the limits of quantification ranged from 0.08 to 3.62 mg/L.The precision experiment results showed that the relative standard deviation(RSD)was less than 3%,the average recoveries of spiked samples ranged from 95.49%to 109.87%.Conclusion This method is simple,rapid and highly accurate,making it suitable for the qualitative and quantitative analysis of the functional components in the aforementioned 7 kinds of medicinal and food homologous substances.

关 键 词：高效液相色谱法 同时测定 药食同源 功能成分 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]