Fine mapping of Pm71,a novel powdery mildew resistance gene from emmer wheat  

作　　者：Jiadong Zhang Hong Yang Guohao Han Hongxing Xu Ruishan Liu Ningning Yu Ran Han Yaoxue Li Jiatong Li Yintao Dai Cheng Liu Yuli Jin Pengtao Ma 

机构地区：[1]Yantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization,College of Life Sciences,Yantai University,Yantai 264005,Shandong,China [2]Guangxi Key Laboratory of Agro-Environment and Agro-Products Safety,Key Laboratory of Crop Cultivation and Physiology,College of Agriculture,Guangxi University,Nanning 530004,Guangxi,China [3]Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang 050021,Hebei,China [4]School of Life Sciences,Henan University,Kaifeng 475004,Henan,China [5]Crop Research Institute,Shandong Academy of Agricultural Sciences/National Engineering Research Center for Wheat and Maize/National Key Laboratory of Wheat Breeding/Key Laboratory of Wheat Biology and Genetic Improvement in the North Huang-Huai River Valley/Shandong Wheat Technology Innovation Center,Jinan 250100,Shandong,China

出　　处：《The Crop Journal》2025年第1期62-68,共7页作物学报(英文版)

基　　金：financially supported by National Natural Science Foundation of China(32301800,32301923 and 32072053);Wheat Industrial Technology System of Shandong Province(SDAIT-01-01);Key Research and Development Project of Shandong Province(2022LZG002-4,2023LZGC009-4-4).

摘　　要：Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.

关 键 词：Blumeria graminis BSE-Seq Disease resistance Fine mapping Triticum dicoccum 

分 类 号：S512.1[农业科学—作物学]