Puccinia triticina effector Pt-1234 modulates wheat immunity by targeting transcription factor TaNAC069 via its C subdomain  

作　　者：Huaimin Geng Yanjun Zhang Zhen Qin Shen Wang Changshan Liu Zhongchi Cui Daqun Liu Haiyan Wang 

机构地区：[1]College of Plant Protection,Hebei Agricultural University/Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province,Baoding 071000,Hebei,China [2]Department of Resource and Environmental Engineering,Xingtai Polytechnic College,Xingtai 054000,Hebei,China [3]Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences/Hebei Laboratory of Crop Genetic and Breeding,Shijiazhuang 050035,Hebei,China

出　　处：《The Crop Journal》2025年第1期69-78,共10页作物学报(英文版)

基　　金：funded by State Key Laboratory of North China Crop Improvement and Regulation(NCCIR2023ZZ-10);the National Natural Science Foundation of China(32172384 and 31501623);the Natural Science Foundation of Hebei(C2020204028);the Science and Technology Research Project of Higher Education of Hebei(ZC2023178).

摘　　要：The NAC(NAM,ATAF1/2,and CUC2)is a defense-associated transcription factor(TF)family that positively regulates defense responses to pathogen infection.TaNAC069 positively regulates resistance in wheat to Puccinia triticina(Pt).However,the molecular mechanism of its interaction with a Pt effector is not clear.We found that Pt effector Pt-1234 interacts with TaNAC069 to subvert host immunity during Pt infection.Quantitative real-time PCR analysis showed that expression of Pt-1234 was significantly upregulated during the early stage of Pt infection.Protein-mediated cell death assays in wheat showed that the Pt-1234 protein was unable to induce cell death in wheat near-isogenic lines carrying different leaf rust resistance genes,whereas it suppressed BAX-induced cell death in leaves of Nicotiana benthamiana.Silencing of Pt-1234 by host-induced gene silencing(HIGS)significantly reduced the virulence of Pt in the susceptible wheat variety Thatcher.The C subdomain of TaNAC069 was responsible for its interaction with Pt-1234,and the E subdomain was required for TaNAC069-mediated defense responses to Pt in planta.These findings indicate that Pt utilizes Pt-1234 to interact with wheat transcription factor TaNAC069 through its C subdomain,thereby modulating wheat immunity.

关 键 词：EFFECTORS NAC transcription factor Host-induced gene silencing Puccinia triticina Triticum aestivum 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]