WRKY10 and ABF1/2 bind to VQ8 to form an accelerator-brake module for the regulation of dark-and ABA-induced leaf senescence in rice  

作　　者：Sique Chen Xianfeng Yang Hongrui Cao Baolin Huang Xiujuan Zheng Wenjia Xie Kangjing Liang Xinli Sun 

机构地区：[1]Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China [2]Key Laboratory of Crop Breeding in South Zhejiang,Key Laboratory of Wenzhou Endemic Crop Biotechnology Innovation,Wenzhou Vocational College of Science&Technology/Wenzhou Academy of Agricultural Sciences,Wenzhou 325006,Zhejiang,China

出　　处：《The Crop Journal》2025年第1期145-157,共13页作物学报(英文版)

基　　金：supported by the National Natural Science Foundation of China (31371557 and 31571574);Wenzhou Basic Scientific Research Project (N20240009)。

摘　　要：Transcription factors(TFs)play key roles in the regulatory network of leaf senescence.However,many nodes in this network remain unclear.To elucidate the mechanism of leaf senescence mediated by a rice TF,WRKY10,the expression of multiple senescence-related genes and physiological phenotypes were monitored in WRKY10-and VQ MOTIF-CONTAINING PROTEIN8(VQ8)-overexpressing plants and the wrky10 and vq8 mutants.Our results showed that WRKY10 positively regulates abscisic acid(ABA)-and dark-induced senescence(DIS)by directly regulating the expression of multiple senescence-related genes.The VQ8 protein,a repressor of WRKY10,negatively regulates WRKY10-mediated DIS.The WRKY10-VQ8 module fine-tunes the progression of DIS.ABA,methyl jasmonate,and H_(2)O_(2) accelerate WRKY10-mediated DIS,whereas ammonium nitrate and dithiothreitol delay WRKY10-mediated DIS.Further analysis revealed that WRKY10 and VQ8 interact with ABA RESPONSIVE ELEMENT BINDING FACTOR1(ABF1)or ABF2.VQ8 represses the transcriptional activity of ABF1 and ABF2.Overexpression of ABF1 or ABF2 accelerates ABA-and dark-induced senescence and H_(2)O_(2) accumulation in N.benthamiana leaves,and WRKY10 and VQ8 can inhibit either ABF1-or ABF2-induced cell necrosis.Taken together,WRKY10 integrates multiple senescence signals to establish an orderly progression of leaf senescence.The VQ8 protein acts as a brake on WRKY10-induced senescence and ABF1/2-induced cell death,preventing uncontrolled cell death.

关 键 词：WRKY10-VQ8 module DARKNESS ABA Leaf senescence Oryza sativa 

分 类 号：S511[农业科学—作物学]