Integrated multi-omics reveals contrasting epigenetic patterns in leaf and root morphogenesis in Aegilops speltoides  

作　　者：Lingfeng Miao Zhe Chen Lifeng Gao Jizeng Jia Weilong Guo Guangyao Zhao 

机构地区：[1]Key Laboratory of Crop Gene Resources and Breeding,the National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Frontiers Science Center for Molecular Design Breeding,Key Laboratory of Crop Heterosis and Utilization,Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China

出　　处：《The Crop Journal》2025年第1期292-298,共7页作物学报(英文版)

基　　金：supported by the National Key Research and Development Program of China(2023YFD1200403).

摘　　要：Aegilops speltoides,the closest ancestor of the wheat B subgenome,has been well studied genomically.However,the epigenetic landscape of Ae.speltoides and the effects of epigenetics on its growth and development remain poorly understood.Here,we present a comprehensive multi-omics atlas of leaves and roots in Ae.speltoides,encompassing transcriptome,DNA methylation,histone modifications,and small RNA profiling.Divergent DNA methylation levels were detected between leaves and roots,and were associated with differences in accumulated 24-nt siRNAs.DNA methylation changes in promoters and gene bodies showed strong connections with altered expression between leaves and roots.Transcriptional regulatory networks(TRN)reconstructed between leaves and roots were driven by tissue-specific TF families.DNA methylation and histone modification act together as switches that shape root and leaf morphogenesis by modulating the binding of tissue-specific TFs to their target genes.The TRNs in leaves and roots reshaped during wheat polyploidization were associated with alterations in epigenetic modi-fications.Collectively,these results not only shed light on the critical contribution of epigenetic regulation in the morphogenesis of leaves and roots in Ae.speltoides but also provide new insights for future investigations into the complex interplay of genetic and epigenetic factors in the developmental biology of common wheat.

关 键 词：Aegilops speltoides DNA methylation Histone modification Transcriptional regulatory network 

分 类 号：S512.1[农业科学—作物学]