基于转录组解析两种砧木嫁接灵武长枣果实品质形成的遗传特性  

Genetic Characterization of Fruit Quality Formation in Lingwuchangzao Jujube Grafted on Two Rootstocks Based on Transcriptome Analysis

作　　者：李敬祖 王博涛 张磊[1] 吴凯玲 马亚平 宋丽华[1] LI Jingzu;WANG Botao;ZHANG Lei;WU Kailing;MA Yaping;SONG Lihua(School of Forestry and Prataculture,Ningxia University,Yinchuan,Ningxia 750021)

机构地区：[1]宁夏大学林业与草业学院,宁夏银川750021

出　　处：《核农学报》2025年第4期707-718,共12页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(32160360)。

摘　　要：为探究不同砧木嫁接灵武长枣果实品质形成的遗传特性,本研究以酸枣和青枣为砧木的3年生嫁接灵武长枣为试验对象,测定两种嫁接灵武长枣的生长和果实品质指标,利用转录组联合WGCNA分析果实品质,构建与果实纵横径和可溶性糖相关的基因模块,筛选调控果实品质的关键基因。结果表明,以青枣为砧木的嫁接灵武长枣生长势更强,但果实品质显著劣于以酸枣为砧木的嫁接灵武长枣。转录组测序结合WGCNA分析筛选到与果实品质指标高度关联的‘honeydew1’模块,获得模块中差异表达基因1 004个,其中上调基因512个、下调基因492个。KEGG分析表明差异表达基因主要富集在植物激素信号转导途径、淀粉和蔗糖代谢途径、苯丙素的生物合成途径,牛胆素和亚牛磺酸代谢富集因子最大。筛选到调控糖及有机酸代谢、木质素代谢的关键差异基因FRK5、HK2、4CL、COMT、BBE等,两种砧木通过改变灵武长枣接穗基因的表达,进一步影响接穗的生长和果实品质。本研究为改良灵武长枣的生长发育状况与果实品质提供了理论依据。To investigate the genetic characteristics of fruit quality formation in grafted Lingwuchangzao(Ziziphus jujuba Mill cv.Lingwuchangzao)using different rootstocks,this study investigated 3-year-old grafted Lingwuchangzao on wild jujube(Ziziphus jujuba Mill.var.spinosa)and Yunnan spiny jujube(Ziziphus mauritiana Lam).The growth and fruit quality traits of the two grafted Lingwuchangzao were measured.Using transcriptomic analysis combined with weighted gene co-expression network analysis(WGCNA),we analyzed fruit quality,constructed a gene co-expression network related to fruit size and soluble sugar content,and identified key genes regulating fruit quality.The results indicated that Lingwuchangzao grafted onto the Yunnan spiny jujube rootstock exhibited greater growth potential,but had significantly inferior fruit quality compared to those grafted onto the wild jujube rootstock.Transcriptome sequencing and WGCNA identified the'honeydew1'module,which was highly associated with fruit quality indicators.Gene Ontology(GO)enrichment analysis revealed 1004 differentially expressed genes,including 512 upregulated and 492 downregulated genes.Kyoto Encyclopedia of Genes and Genomes(KEGG)indicated that the differentially expressed genes were primarily enriched in plant hormone signal transduction,starch and sucrose metabolism,and phenylpropanoid biosynthetic pathways.The most significant enrichments were observed in taurine and hypotaurine metabolism.Key differentially expressed genes,including FRK5,HK2,4CL,COMT,and BBE were identified as regulators of sugar and organic acid metabolism and lignin metabolism.In conclusion,the two rootstocks affected the growth and fruit quality of the scions by altering the expression of Lingwuchangzao scion genes.This study provides a theoretical basis for improving the growth and fruit quality of Lingwuchangzao trees.

关 键 词：灵武长枣 砧木 差异基因 代谢途径 果实品质 

分 类 号：S665.1[农业科学—果树学]