基于3K和48K SNP芯片进行高低种植密度下玉米株高相关性状的作图比较  

Comparing Mapping for Maize Plant Height-Related Traits under High and Low Planting Densities via 3K and 48K SNP Chips

作　　者：李阳婧 杨泽兵 罗洁 池双红 刘应红[2] 易强 侯宪斌 LI Yangjing;YANG Zebing;LUO Jie;CHI Shuanghong;LIU Yinghong;YI Qiang;HOU Xianbin(College of Agriculture,Guizhou University,Guiyang,Guizhou 550025;Maize Research Institute,Sichuan Agricultural University,Chengdu,Sichuan 511130;College of Agriculture and Food Engineering,Baise University,Baise,Guangxi 533000)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]四川农业大学玉米研究所,四川成都511130 [3]百色学院农业与食品工程学院,广西百色533000

出　　处：《核农学报》2025年第4期719-727,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(32260470);贵州省基础研究计划(黔科合基础-ZK[2023]一般113);广西自然科学基金(2021GXNSFBA196069);贵阳市乌当区“六化”玉米制种基地示范项目。

摘　　要：为了比较不同密度单核苷酸多态性(SNP)芯片的数量性状位点(QTL)作图效率,本研究基于Maize SNP 3K和48K芯片对来源于R08×掖478的271个重组自交系(RIL)群体进行基因分型,采用完备区间作图法定位四个环境和两种种植密度条件下玉米株高相关性状的QTL。结果表明,利用48K SNP芯片分型结果构建的遗传图谱分辨率高于3K(标记数:2 804个bin vs 683个SNP;图谱长:3 863.77 cM vs 1 786.06 cM;标记平均间距:1.38 cM vs 2.65 cM)。基于48K-遗传图谱在高低种植密度下定位到的QTL较3K-遗传图谱多近40%(86/62),两者共定位到28个位置相同的QTL,约占48K-遗传图谱检测到QTL总数的32.6%和3K-遗传图谱检测到QTL总数的45.2%。利用48K-遗传图谱在高种植密度下检出51个QTL,低种植密度下检出60个QTL,其中密度钝感型QTL为25个。基于高密度标记芯片作图具有更高的效率,可用于高效解析玉米数量性状。本研究结果为玉米耐密候选基因的进一步精细定位提供了理论支撑。To evaluate the efficiency of quantitative trait locus(QTL)mapping using different singlenucleotide polymorphism(SNP)chips,this study used a population of 271 recombinant inbred lines(RILs)derived from a cross between the inbred lines R08 and Ye478.Using the complete interval mapping method,these lines were genotyped with the Maize SNP 3K and 48K chips to identify QTL associate with maize plant height-related traits across four environments and under two planting densities.The findings indicated that the genetic map constructed with the 48K SNP chip exhibited greater resolution compared to the genetic map generated with the 3K chip.This disparity is illustrated by several metrics,including the number of markers(2804 bins with the 48K SNP chip compared to 683 SNPs with the 3K SNP chip),the length of the genetic map(3863.77 cM versus 1786.06 cM),and the average genetic interval between adjacent markers(1.38 cM versus 2.65 cM).The genetic map constructed using the 48K SNP chip identified nearly 40%more QTLs than the map generated with the 3K chip,detecting 86 QTLs identified with the high-density map,compared to 62 QTLs with the 3K chip.Additionally,28 QTLs were found to be co-located in the genetic maps derived from both the 3K and 48K SNP chips under high and low planting densities,representing approximately 32.6%of the total QTLs detected by the 48K chip,while 45.2%were identified by the 3K chip genetic map.Specifically,the genetic map generated with the 48K SNP chip detected 51 QTLs under high planting density and 60 QTLs under low planting density.Among these,25 QTLs were detected across both planting densities.In conclusion,QTL mapping using a high-density marker chip offers greater mapping resolution and can be effectively utilized for dissecting complex traits in maize.The results of the present study offer support for the subsequent fine mapping of maize candidate genes associated with high-planting-density tolerance.

关 键 词：高密度基因芯片 玉米株高 耐密性 QTL作图分辨率比较 

分 类 号：S513[农业科学—作物学]