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作 者:黎洁华 王倩倩 何玉华 赵爽[1] 刘晔 张飞[1] 陈发棣[1] 房伟民[1] LI Jiehua;WANG Qianqian;HE Yuhua;ZHAO Shuang;LIU Ye;ZHANG Fei;CHEN Fadi;FANG Weimin(College of Horticulture,Nanjing Agricultural University/State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilisation/Key Laboratory of Flower Biology and Germplasm Enhancement(South),Ministry of Agriculture and Rural Affairs/Key Laboratory of Biology of Ornamental Plants in East China,National Forestry and Grassland Administration/Zhongshan Biological Breeding Laboratory,Nanjing,Jiangsu 210095)
机构地区:[1]南京农业大学园艺学院/作物遗传与种质创新利用全国重点实验室/农业农村部花卉生物学与种质创制重点实验室(南方)/国家林业和草原局华东地区花卉生物学重点实验室/钟山生物育种实验室,江苏南京210095
出 处:《核农学报》2025年第4期728-736,共9页Journal of Nuclear Agricultural Sciences
基 金:江苏种业振兴揭榜挂帅项目(JBGS[2021]094);江苏现代农业产业技术体系建设项目(JATS[2023]417);江苏省林业科技创新与推广项目(LYKJ[2021]13)。
摘 要:为探索菊花黑斑病病原菌最佳的体外产孢条件,以致病菌链格孢菌(Alternaria alternata)CJ3为供试菌株,研究了温度、光照时间、培养时间和紫外照射时间等单因素变化对病菌产孢量的影响。此外,采用正交试验设计比较该菌株在不同接种温度、接种浓度和接种方式组合下接种,园林小菊灵岩黄离体叶片的发病情况,建立最优接种体系并进行活体接种验证。结果表明,黑斑病病菌CJ3的最佳产孢条件是菌丝损伤后紫外照射15 min,在22℃的全黑暗环境下培养28 d;在接种温度为22℃,孢子悬浮液浓度为1×10^(8) CFU·mL^(-1),接种方法为摩擦法的接种条件下,离体叶片的发病率为93.33%,病情指数为80.33,显著高于其他组合,是园林小菊黑斑病孢子悬浮液接种抗病性鉴定的最佳方法;在活体植株上的发病率为90.00%,病情指数为71.58,离体接种与活体接种病情指数的相关性系数为0.83(P<0.01),说明该接种体系适用于园林小菊离体与活体抗病性鉴定。本研究优化了菊花黑斑病病原菌的产孢条件和孢子悬浮液接种抗病鉴定体系,为产孢诱导、病害防控和抗病品种选育提供了理论依据和技术支撑。To explore the optimal in vitro sporulation conditions for the pathogen causing chrysanthemum black spot disease,Alternaria alternata CJ3 was used as the test strain to study the effects of single factors,including temperature,photoperiod,incubation period and ultraviolet irradiation duration on pathogen sporulation.In addition,an orthogonal experiment was designed to establish the optimal inoculation system by investigating the effects of inoculation temperature,inoculation concentration and inoculation method on isolated leaves of the garden chrysanthemum Lingyan Huang.The optimal inoculation system was subsequently verified on living plants.The results showed that the optimal sporulation conditions of CJ3 were irradiating with ultraviolet light for 15 min after mycelial damage and incubating at 22℃in complete darkness for 28 d.When isolated leaves of the garden chrysanthemum were inoculated by rubbing them with spore suppension of 1×10^(8)CFU·mL^(-1)and placed under 22℃,the incidence rate reached 93.33%and the disease index was 80.33,significantly higher than that of other treatments.These suggested that inoculating with spore suspension was the most effective method for identifying resistant to black spot disease in garden chrysanthemum.The incidence rate on the living plants was 90.00%,and the disease index was 71.58.The correlation coefficient between in vitro and in vivo inoculations was 0.83(P<0.01),indicating this inoculation system was suitable for both in vitro and in vivo identification of disease resistance in garden chrysanthemums.This study optimized the sporulation conditions of the pathogen causing chrysanthemum black spot disease and the disease resistance identification system using spore suspension inoculation,which will provide references and technical support for sporulation induction,disease prevention and control,and disease-resistant breeding.
分 类 号:S436.8[农业科学—农业昆虫与害虫防治]
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