菊属植物PIN基因家族的鉴定及生物信息学分析  

Identification and bioinformatics analysis of PIN gene family in chrysan themum plants

作　　者：刘波 刘欣宇 李骏倬 武晓云 卢敏 王佳颖 戴思兰 LIU Bo;LIU Xinyu;LI Junzhuo;WU Xiaoyun;LU Min;WANG Jiaying;DAI Silan(Beijing Laboratory for Urban and Rural Ecological Environment/Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding/National Engineering Research Center for Floriculture,School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学园林学院,城乡生态环境北京实验室/花卉种质创新与分子育种北京市重点实验室/国家花卉工程技术研究中心,北京100083

出　　处：《浙江大学学报(农业与生命科学版)》2025年第1期39-53,共15页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金项目(32371948)。

摘　　要：PIN蛋白家族是生长素外排载体,负责将生长素从细胞内向细胞外运输,这一过程对于植物体内生长素的分布和浓度梯度的建立至关重要。本研究采用生物信息学手段,在5个菊属植物中鉴定出113个PIN基因家族成员,并深入分析了它们的蛋白理化性质,揭示了这些成员在物理和化学特性上的多样性。通过构建系统进化树发现,PIN基因被分为6个不同的分支,且亲缘关系相近的成员在保守基序和保守结构域上具有相似性,暗示它们可能具有相似或相关的功能。染色体定位和共线性分析进一步展示了PIN基因在菊属植物中的分布模式,以及它们在基因组中的复制和重排事件。此外,启动子顺式作用元件分析显示,PIN基因的启动子区域富含与生长发育、激素信号传导和逆境响应相关的调控元件,表明PIN基因可能在植物生长发育和环境适应过程中受到多种信号的调控。基因表达分析发现,PIN基因在菊花不同器官中具有特异性表达模式。本研究结果为后续探究菊属植物中PIN基因的功能奠定了理论基础。The PIN protein family is an auxin efflux carrier responsible for transporting auxin from intracellular to extracellular space,a process that is essential for the distribution of auxin and the establishment of concentration gradients in plants.In this study,113 members of the PIN gene family were identified in five species of chrysanthemum plants by bioinformatics methods,and their physicochemical properties were analyzed in depth,revealing the diversity of physical and chemical properties of these members.By constructing a phylogenetic tree,we found that these PIN genes were divided into six distinct branches,and the closely related members presented similar conserved motifs and conserved domains,suggesting that they may have similar or related functions.Chromosome localization and collinearity analysis further demonstrated the distribution patterns of PIN genes in chrysanthemum plants,as well as their replication and rearrangement events in the genome.In addition,promoter cis-acting element analysis showed that the promoter region of the PIN gene was rich in regulatory elements related to growth and development,hormone signal transduction and stress response,suggesting that the PIN gene may be regulated by multiple signals in plant growth and development and environmental adaptation.Gene expression analysis showed that the PIN gene presented specific expression patterns in different organs of chrysanthemum plants.The above results provide a theoretical basis for the subsequent study of the function of the PIN gene in chrysanthemum plants.

关 键 词：PIN基因家族 菊属植物 生物信息学分析 

分 类 号：S682.11[农业科学—观赏园艺]