幽门螺杆菌杀菌试验方法的建立及其对常用消毒剂抗力的研究  

Study on establishment of bactericidal test method for Helicobacter pylori and its resistance to commonly used disinfectants

作　　者：魏兰芬[1] 潘协商[1] 林洁[2] 谢中艺 蔡冉[1] 许激[1] WEI Lan-fen;PAN Xie-shang;LIN Jie;XIE Zhong-yi;CAI Ran;XU Ji(Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China;Sir Run Run Shaw Hospital,Zhejiang University School of Medicine)

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051 [2]浙江大学医学院附属邵逸夫医院

出　　处：《中国消毒学杂志》2025年第1期1-3,共3页Chinese Journal of Disinfection

摘　　要：目的建立幽门螺杆菌悬液定量杀菌试验方法,并测定其对常用消毒因子的抗力。方法以悬液定量杀菌试验方法为基础,建立幽门螺杆菌杀灭试验方法并观察其对常用消毒剂的抗力。结果用含8%新生牛血清的布氏琼脂为培养基,采用涂抹接种法、微需氧条件培养,适用于幽门螺杆菌的活菌计数培养。悬液定量杀菌试验结果表明,幽门螺杆菌对次氯酸钠、碘伏、葡萄糖酸氯己定和季铵盐等常用消毒剂的抗力略低于大肠埃希菌标准株。结论本研究建立的幽门螺杆菌悬液定量杀灭试验条件可用于常用消毒因子杀灭该细菌的效果评价方法。Objective To establish a suspension quantitative bactericidal test method for Helicobacter pylori so as to determine its resistance to commonly used disinfection factors.Methods Based on the suspension quantitative bactericidal test method,a method for killing Helicobacter pylori was established and its resistance to commonly used disinfectants was observed.Results Brucella agar containing 8%newborn bovine serum was used as culture medium,and the smear inoculation method and microaerobic conditions were used for viable bacterial count culture of Helicobacter pylori.The suspension quantitative test results showed that Helicobacter pylori had slightly lower resistance to commonly used disinfectants such as sodium hypochlorite,iodophor,chlorhexidine gluconate,and quaternary ammonium salts than the standard strain of Escherichia coli.Conclusion The suspension quantitative bactericidal test method for Helicobacter pylori established in this study can be used as a method for evaluating the effectiveness of commonly used disinfection factors in killing this bacterium.

关 键 词：幽门螺杆菌 大肠埃希菌 消毒因子 杀菌试验 

分 类 号：R187.2[医药卫生—流行病学]