过氧乙酸消毒剂对生物膜的去除效果观察  

Observation on the removal effect of peracetic acid disinfectant on the biofilm

作　　者：林曼婷[1] 苏裕心 巩爱华 申仲翔 陈睿 LIN Man-ting;SU Yu-xin;GONG Ai-hua;SHEN Zhong-xiang;CHEN Rui(Guangdong Medical Devices Quality Surveillance and Test Institute,Guangzhou 510663,China;Zhongguancun International Medical Inspection and Certification Co.,Ltd.)

机构地区：[1]广东省医疗器械质量监督检验所,广东广州510663 [2]中关村国际医药检验认证科技有限公司

出　　处：《中国消毒学杂志》2025年第1期13-15,共3页Chinese Journal of Disinfection

摘　　要：目的观察内镜再处理过程中常用的过氧乙酸消毒剂对生物膜的去除效果。方法使用铜绿假单胞菌和聚四氟乙烯管腔,培养5 d以制备生物膜。其后用不同浓度的过氧乙酸溶液冲洗生物膜5、10、30和60 min,观察其对生物膜的去除效果。结果仅2000 mg/L过氧乙酸溶液处理生物膜60 min后,载体上细菌的杀灭对数值为3.62,其他平均杀灭对数值均<3.00。结论过氧乙酸消毒液无法充分去除聚四氟乙烯管腔内的生物膜,当生物膜在内镜内积聚时,未清洗直接用过氧乙酸处理会导致消毒失败。Objective To verify the removal effect of commonly used peracetic acid disinfectant on biofilms during endoscopic reprocessing.Methods Pseudomonas aeruginosa and polytetrafluoroethylene tubes were used to culture for 5 d to prepare biofilm,and then the biofilm was rinsed with different concentrations of peracetic acid solution for 5,10,30 and 60 min to observe its removal effect on biofilm.Results After the biofilm was treated with 2000 mg/L peracetic acid solution for 60 min,the killing log value of bacteria on the carrier was 3.62,while the average killing log values for other concentrations of peracetic acid solution were all<3.00.Conclusion Peracetic acid disinfectant cannot fully remove biofilm inside polytetrafluoroethylene lumen.When the biofilm accumulates inside the endoscope,direct treatment with peracetic acid without cleaning may lead to disinfection failure.

关 键 词：内镜 消毒剂 过氧乙酸 生物膜 去除 

分 类 号：R187.1[医药卫生—流行病学]