基于数据挖掘的过敏性哮喘动物模型应用分析  

Analysis of Animal Models of Allergic Asthma Based on Data Mining

作　　者：吴涵 胡志祥 吉美奇 尹浩 吕玉娥 翟春涛 WU Han;HU Zhixiang;JI Meiqi;YIN Hao;LYU Yu'e;ZHAI Chuntao(Shanxi University of Chinese Medicine,Jinzhong 030600,China;Shanxi Acupuncture Hospital,Taiyuan 030006,China)

机构地区：[1]山西中医药大学,山西晋中030600 [2]山西省针灸医院,太原030006

出　　处：《中国实验方剂学杂志》2025年第5期211-217,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院科技创新工程项目(CI2022E010XB);山西省科技人才创新团队建设项目(202204051002011);山西省中医药管理局科研课题(2023ZYYB047);山西中医药大学创新团队项目(2022TD1006);吕景山国医大师“对法”传承省技术创新中心项目(2022D100089052823242891545041);山西省医学重点科研项目(2022XM35)。

摘　　要：目的:通过统计分析过敏性哮喘动物模型的造模特点和指标选择,为建立理想的过敏性哮喘动物模型提供参考依据。方法:使用计算机从中国知网、重庆维普、万方、中国生物医学文献数据库及PubMed数据库搜索资源,选择“过敏性哮喘”作为主题词,搜索范围为2019年1月到2024年1月。通过整合文献和提取数据,使用Excel 2021创建个人数据库,对实验所选择的动物品系、性别、致敏物质、模型制备途径、检测指标及检测方法等进行梳理,并运用Excel 2021、SPSS Modeler 18.0、Cytoscape 3.10.2对过敏性哮喘动物模型的相关特性进行分析。结果:数据库共纳入418篇文献,经过对比分析显示造模时应用最多的动物品系为BALB/c小鼠;性别以雌性为主;造模方法以卵清蛋白(OVA)腹腔注射致敏,配合雾化激发给药为主;检测指标主要选择表观指征、细胞分析、肺组织病理、肺功能检测、肺组织蛋白表达、肺组织基因表达等;检测方式主要涉及病理学染色、酶联免疫吸附测定法、免疫组化、免疫荧光、蛋白印迹法及多种聚合酶链式反应等。结论:过敏性哮喘动物模型目前无公认的造模方法及评价标准,基于数据分析的结果,推荐OVA诱导的BALB/c小鼠过敏性哮喘模型,评价造模是否成功的主要标准为一般行为学改变、肺组织气道形态学改变及炎性细胞浸润情况、血清中促炎因子和抑炎因子及支气管肺泡灌洗液中炎性细胞的改变情况。Objective:To provide a basis for the establishment of an ideal animal model of allergic asthma by statistically analyzing the modeling characteristics and the selection of indicators of the available models.Methods:We retrieved the relevant articles from China National Knowledge Infrastructure(CNKI),VIP,Wanfang Data,SinoMed,and PubMed with"allergic asthma"as the keyword and the time interval from January 2019 to January 2024.Through integrating the literature and extracting data,we used Excel 2021 to create a personal database and sorted out the animal strains,genders,allergenic substances,modeling routes,and test indicators and methods.Excel 2021,Cytoscape 3.10.2,and SPSS Modeler 18.0 were then used to analyze the relevant characteristics of the animal models.Results:A total of 418 articles were included in the database,and the comparative analysis showed that the most frequently used animal strain for modeling was BALB/c mice,and female animals were mostly used.The main modeling method was sensitization by intraperitoneal injection of ovalbumin(OVA),which was combined with intranasal inhalation.The test indicators mainly included appearance signs,cellular analysis,lung histopathology,lung function indicators,and protein and gene expression in the lung.The test methods mainly involved pathological staining,enzyme-linked immunosorbent assay,immunohistochemistry,immunofluorescence,Western blot,and polymerase chain reaction(PCR)assays.Conclusion:There is no recognized modeling method or evaluation standard for the animal models of allergic asthma.Based on the results of data analysis,the OVA-induced allergic asthma model in BALB/c mice is recommended.The main criteria for evaluating the success of modeling are the general behavioral changes,the morphological changes of the airway and inflammatory cell infiltration in the lung tissue,the changes of pro-inflammatory and anti-inflammatory cytokines in the serum,and the alterations of inflammatory cells in the bronchoalveolar lavage fluid.

关 键 词：过敏性哮喘 数据挖掘 动物模型 应用分析 检测指标 

分 类 号：R285.5[医药卫生—中药学] R289[医药卫生—中医学] R256.1