不同抗性柑橘品种感染柑橘轮斑病菌后防御酶活性的变化  

Changes of defense enzyme activity in different resistant citrus varieties infected by Pseudofabraea citricarpar

作　　者：陈泉 徐嵩琳 何锦辉 刘忠贤 Chen Quan;Xu Songlin;He Jinhui;Liu Zhongxian(Chongqing Three Gorges Academy of Agricultural Science,404155;Economic Crop Development Center in Wanzhou District of Chongqing City)

机构地区：[1]重庆三峡农业科学院,404155 [2]重庆市万州区经济作物发展中心

出　　处：《中国果树》2025年第2期92-98,107,共8页China Fruits

基　　金：重庆市教育委员会科学技术研究项目(KJ202301289858551);重庆市万州区科研项目(wzstc-20230201)。

摘　　要：为研究感染轮斑病菌后柑橘叶片相关防御酶活性的变化,揭示不同抗性品种对柑橘轮斑病的抗病生理机制提供理论依据,以采集的高感品种尤力克柠檬、福本脐橙,中抗品种塔罗科血橙新系、伦晚脐橙和高抗品种沙田柚、血橙8号6个柑橘品种叶片为材料,离体接种轮斑病(Pseudofabraea citricarpar)菌株WZSS1后在0、2、5、10、15、20、30 d取样,采用分光光度法测定其过氧化氢酶(CAT)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)、多酚氧化酶(PPO)活性,分析病菌侵染对不同抗性柑橘品种酶活性的影响。结果表明:接种轮斑病菌后,高感品种尤力克柠檬、福本脐橙的叶片CAT活性均在接种后2 d达到最高值,分别比接种后0 d时提升了417.59%和110.60%,且均显著高于其他供试品种;高抗品种的叶片CAT活性在接种后30 d达到最高值,且均显著高于其他供试品种。尤力克柠檬叶片POD活性有3个高峰,分别出现在接种后0、10、20 d,且均显著高于其他供试品种;高抗品种叶片POD活性在接种后2 d快速降低,接种后30 d的POD活性均显著高于其他供试品种。高感品种、中抗品种叶片PAL活性均表现出先下降后上升的趋势;高感品种、高抗品种叶片PAL活性在接种后10 d降至最低值,中抗品种在接种后15 d达到最低值。高感品种叶片SOD活性均在接种后2 d骤然升高;中抗品种、高抗品种叶片SOD活性最高值出现的时间比高感品种晚3 d以上。在接种后2 d,供试品种的叶片PPO活性均增加;之后,不同品种表现出不同的变化规律,抗病品种的叶片PPO活性最高值出现时间明显晚于高感品种,抗性越强的品种,峰值也越高。综上,高感品种接种柑橘轮斑病菌后,其叶片CAT活性在接种后2 d达到最高值,且显著高于供试的抗病品种;高抗品种的叶片CAT活性在接种后30 d达到最高值,且显著高于其他品种;抗病品种叶片SOD、PPO活性最�To study the changes of related defense enzyme activities of leaves of citrus which was infected by Pseudofabraea citricarpar,and to provide theoretical basis for revealing the physiological mechanism of different resistant varieties to P.citricarpar,the leaves of 6 citrus varieties including high-sensitivity varieties such as‘Eureka’lemon and‘Fukumoto’navel orange,medium-resistant varieties such as‘Tarocco blood orange nucellar line’and‘Lane late’navel orange,and high-resistance varieties such as‘Shatianyou’and‘Blood orange 8’were collected as materials.After in vitro inoculation with P.citricarpar strain WZSS1,samples were taken at 0 day,2 days,5 days,10 days,15 days,20 days and 30 days,and the activities of catalase(CAT),peroxidase(POD),phenylalanine ammonia lyase(PAL),superoxide dismutase(SOD),and polyphenol oxidase(PPO)were measured using spectrophotometry to analyze the effect of pathogen infection on enzyme activity in different resistant citrus varieties.The results showed that after inoculation with P.citricarpar,the CAT activity of high-sensitivity varieties such as‘Eureka’lemon and‘Fukumoto’navel orange reached the maximum at 2 days after inoculation,increased by 417.59%and 110.60%compared with 0 day after inoculation,respectively,and was significantly higher than other tested varieties;the CAT activity of high-resistance varieties reached its highest value at 30 days after inoculation,and was significantly higher than other tested varieties.The POD activity in leaves of‘Eureka’lemon had three peaks at 0 days,10 days and 20 days after inoculation,and were significantly higher than other tested varieties;the POD activity of high-resistant varieties decreased rapidly at 2 days after inoculation,and the POD activity at 30 days after inoculation was significantly higher than that of other tested varieties.The PAL activity in leaves of high-sensitivity and medium-resistant varieties showed a trend of first decreasing and then increasing;the PAL activity in the leaves of h

关 键 词：柑橘 轮斑病 抗性 防御酶 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]