天麻素及电针对局灶性脑缺血大鼠尾壳核GAP-43和SYP表达的影响  

Effect of gastrodin and electro-acupuncture on expression of growth-associated protein 43 and synaptophysin in caudate putamen of focal cerebral ischemia rats

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作  者:李晨妤 聂泽银 缪化春[1] 吴锋[1] LI Chengyu;NIE Zeyin;MIAO Huachun;WU Feng(Department of Human Anatony,Wannan Medical College,Wuhu 241002,China)

机构地区:[1]皖南医学院人体解剖学教研室,安徽芜湖241002

出  处:《包头医学院学报》2025年第2期35-38,81,共5页Journal of Baotou Medical College

基  金:皖南医学院重点科研项目(No.WK2019Z05);皖南医学院学术带头人后备人选人才项目(No.校政[2019]81号)。

摘  要:目的:观察天麻素结合电针对局灶性脑缺血大鼠尾壳核生长相关蛋白43(growth-associated protein,GAP-43)和突触素(synaptophysin,SYP)蛋白表达是否具有协同增效作用,探讨针药结合治疗脑缺血的部分神经生物学机制。方法:48只雄性成年Sprague-Dawley(SD)大鼠,随机分为正常组、假手术组、模型组、天麻素组、电针组、天麻素+电针组。采用右侧大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)法复制FCI模型。造模成功后2周,天麻素组、电针组和天麻素+电针组大鼠分别给予天麻素注射液、电针及天麻素结合电针连续治疗2周。采用免疫组织化学法检测大鼠尾壳核中GAP-43和SYP蛋白的表达情况。结果:各组大鼠尾壳核中均有GAP-43和SYP蛋白表达。与正常组比较,假手术组大鼠尾壳核中GAP-43和SYP蛋白的表达差异无统计学意义(P>0.05);模型组GAP-43蛋白的表达增加,SYP蛋白的表达减少,差异均有统计学意义(P<0.05)。与模型组比较,天麻素组、电针组和天麻素+电针组大鼠尾壳核中GAP-43和SYP蛋白的表达均增加,差异有统计学意义(P<0.05)。天麻素+电针组大鼠尾壳核中GAP-43和SYP蛋白的表达较天麻素组、电针组增加,差异有统计学意义(P<0.05)。结论:天麻素结合电针可上调局灶性脑缺血大鼠尾壳核中GAP-43和SYP蛋白的表达,这可能有助于促进神经元损伤的修复,且针药结合治疗的效果优于单独天麻素和电针治疗。Objective:To explore the neurobiological mechanism of acupuncture combined with medicine in the treatment of cerebral ischemia by observing the synergistic effect of gastrodin combining with electro-acupuncture(EA)on the expression of growth associated protein 43(GAP-43)and synaptophysin(SYP)in caudate putamen nucleus of rats with focal cerebral ischemia(FCI).Methods:A total of 48 Sprague-Dawley(SD)rats were randomly divided into 6 groups(normal group,sham operation group,model group,gastrodin group,EA group and gastrodin+EA group).The model was prepared by middle cerebral artery occlusion(MCAO).2 weeks after modeling,rats were given gastrodin,EA,or gastrodin+EA for continuous treatment in 2 successive weeks.Then expressions of GAP-43 and SYP protein in the caudate putamen nucleus were detected by immunohistochemistry.Results:Positive expression of GAP-43 and SYP protein was found in each group of rats.Expressions of GAP-43 in model group was higher than the normal group and sham operation group(P<0.05),expressions of SYP in model group was lower than the normal group and sham operation group(P<0.05).Compared with the model group,the positive expressions of GAP-43 and SYP in gastrodin group,the EA group,and the gastrodin+EA group increased significantly(P<0.05).The positive expressions of GAP-43 and SYP increased obviously in the gastrodin+EA group compared to those of the EA group and the medication group(P<0.05).Conclusion:Gastrodin administration and EA intervention can up-regulated the expressions of GAP-43 and SYP protein in the caudate putamen nucleus of FCI rats,which may contribute action in promoting the repair of injured neurons.The effect of gastrodin+EA is better than simple gastrodin or EA treatment.

关 键 词:脑缺血 生长相关蛋白43 突触素 尾壳核 电针 天麻素 

分 类 号:R245[医药卫生—针灸推拿学]

 

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