复方甘芪止咳颗粒组方的提取工艺优化研究  

Optimization of the extraction process of the formulation of Compound Ganqi Anti-Cough Granules

作　　者：刘晓艳 雷文娟 王萌 唐红娟 孟敏[2] 史彦斌[3] LIU Xiaoyan;LEI Wenjuan;WANG Meng;TANG Hongjuan;MENG Min;SHIYanbin(Department of Pharmacy,Jiugang Hospital,Gansu,Jiayuguan 735100,China;Medical Department,Gansu Provincial Hospital,Gansu,Lanzhou 730000,China;College of Pharmacy,Lanzhou University,Gansu,Lanzhou 730000,China;Outpatient Department,Gansu Provincial Hospital,Gansu,Lanzhou 730000,China)

机构地区：[1]甘肃省酒钢医院药剂科,甘肃嘉峪关735100 [2]甘肃省人民医院医务处,甘肃兰州730000 [3]兰州大学药学院,甘肃兰州730000 [4]甘肃省人民医院门诊部,甘肃兰州730000

出　　处：《中国医药科学》2025年第3期74-77,共4页China Medicine And Pharmacy

基　　金：酒钢集团医疗卫生系统科技项目(JG2020YLKJ21)。

摘　　要：目的优化复方甘芪止咳颗粒组方的提取工艺。方法浓氨水引咳法建立小鼠咳嗽模型;高效液相色谱法测定甘草酸铵含量;以乙醇浓度、溶剂用量和提取时间为影响因素,以得膏率、甘草酸铵含量、引咳潜伏时间为评价指标,采用正交实验对复方甘芪止咳颗粒组方的提取工艺进行优化。结果以60%乙醇为溶剂,溶剂体积为药材质量的4倍,煎煮4 h得到的提取物综合评分最高,为复方甘芪止咳颗粒组方的优化提取工艺。结论该提取工艺合理可行,得膏率和甘草酸铵提取率较高,引咳潜伏期长。Objective To optimize the extraction process of the formulation of Compound Ganqi Anti-Cough Granules.Methods A cough model was established in mice using the method of concentrated ammonia induced cough.The content of ammonium glycyrrhizinate was determined by high performance liquid chromatography.The concentration of ethanol,amount of solvent and extraction time were taken as the influencing factors.The extract yielding rate,content of ammonium glycyrrhizinate and the latent period of inducing cough were taken as the evaluation indexes.Results The extract obtained by decocting for 4 hours with 60%ethanol as the solvent and the volume of solvent being 4 times the mass of herbs had the highest overall score,which was the optimized extraction process of Compound Ganqi Anti-Cough Granules.Conclusion The extraction process is reasonable and feasible,with high extract yielding rate,high extraction rate of ammonium glycyrrhizinate,and a long latent period of inducing cough.

关 键 词：复方甘芪止咳颗粒 正交设计 提取工艺 甘草酸铵 咳嗽潜伏期 

分 类 号：R286[医药卫生—中药学]