基于基因及蛋白组学技术探讨HIV/AIDS湿热证的作用机制  

Mechanism of Damp-Heat Syndrome of HIV/AIDS Based on Gene and Proteomics Techniques

作　　者：王娟[1] 张淼[1] 刘飒[1] 马素娜[1] 张海燕[1] 许前磊[2] WANG Juan;ZHANG Miao;LIU Sa;MA Suna;ZHANG Haiyan;XU Qianlei(Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,Henan,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学第一附属医院,河南郑州450000

出　　处：《中华中医药学刊》2025年第2期72-75,I0028,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82004344);中原青年拔尖人才项目(ZYYCYU202012153)。

摘　　要：目的探索艾滋病湿热证分子机制。方法通过Agilent基因表达谱芯片及iTRAQ-MS技术对艾滋病湿热证患者进行基因组学及蛋白组学检测,以比值改变≥1.15或≤0.87,P<0.05为标准共鉴定出人类免疫缺陷病毒(HIV)/艾滋病(AIDS)湿热证差异表达基因20个,差异表达蛋白22个。结果HIV/AIDS湿热证与健康对照组比较,趋化因子受体4(CXCR4)、核转录因子-κB抑制因子(NFκBIA)、Tribbles同源蛋白1(TRIB1)等基因表达增加,趋化因子受体1(CX3CR1)表达降低;血清中载脂蛋白A-Ⅱ(APOA-Ⅱ)、C反应蛋白(CPR)、血小板因子4(PF4)、AAT蛋白表达增多,补体因子B(CFB)、Gelsolin表达降低。结论HIV/AIDS湿热证与炎症反应密切相关,HIV/AIDS湿热证有其特定的分子生物学基础,为中医药客观化和标准化研究提供了方法和依据。Objective To explore the molecular mechanism of damp-heat syndrome in acquired immune deficiency syndrome(AIDS).Method Agilent gene expression profile chip and iTRAQ-MS technology were used to perform genomic and proteomic tests on human immunodeficiency virus(HIV)/AIDS patients with damp-heat syndrome.A total of 20 differentially expressed genes of HIV/AIDS damp-heat syndrome were identified with ratio change≥1.15 or≤0.87 and P<0.05 as the criteria.There were 22 differentially expressed proteins.Results Compared with those of the healthy control group,the expressions of CXCR4,NF-κBIA,TRIB1 and other genes increased,while the expression of CX3CR1 decreased.The expressions of APOA-Ⅱ,CRP,PF4 and AAT were increased,while the expressions of CFB and Gelsolin were decreased.Conclusion HIV/AIDS damp-heat syndrome is closely related to inflammation.HIV/AIDS damp-heat syndrome has its specific molecular biological basis,which provides methods and basis for the objectization and standardization of traditional Chinese medicine.

关 键 词：艾滋病 湿热证 免疫基因 

分 类 号：R259.129.1[医药卫生—中西医结合]