麻杏石甘汤调控TLR4/MyD88/NF-κB信号通路改善哮喘小鼠气道炎症反应的实验研究  

Experimental Study on the Regulation of TLR4/MyD88/NF-κB Signaling Pathway by Maxing ShiganDecoction to Improve Airway Inflammatory Response in Asthmatic Mice

作　　者：肖惠珍 孟媛 周钰 陈仁山 XIAO Huizhen;MENG Yuan;ZHOU Yu;CHEN Renshan(Guangzhou Hospital of Integrated Traditional and Western Medicine,Guangzhou 510800,China)

机构地区：[1]广州市中西医结合医院,广东广州510800

出　　处：《中国民族民间医药》2025年第4期27-32,共6页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：广州市科学技术局(202201011653);广东省中医药局(20231252)。

摘　　要：目的:从TLR4/MyD88/NF-κB信号通路角度探讨麻杏石甘汤治疗哮喘的作用机制。方法:50只雄性BALB/c小鼠随机分为5组,每组10只,分为对照组(Con组),模型组(OVA组),麻杏石甘汤高、低剂量组(MX-H组、MX-L组),地塞米松组(Dex组)。采用卵蛋白腹腔注射和雾化方法建立小鼠哮喘模型,在第22~28天,雾化前1 h分别给予MX-H组、MX-L组麻杏石甘汤12 mg/kg、6 mg/kg灌胃,Dex组给地塞米松(0.5mg/kg)灌胃,Con组予等量生理盐水灌胃。实验完成后处死小鼠,ELISA检测血清和肺泡灌洗液IL-1β、IL-6含量,观察肺组织HE病理变化,RT-qPCR检测小鼠肺组织TLR4、MyD88、NF-κB mRNA表达,Western Blot检测肺组织TLR4、MyD88、NF-κB蛋白表达。结果:造模后,OVA组小鼠与Con组小鼠比较,肺泡灌洗液及血清IL-1β、IL-6浓度显著升高;药物治疗后,Dex组、MX-L组、MX-H组小鼠肺泡灌洗液及血清IL-1β、IL-6浓度均比OVA组明显下降,其中,MX-H组比MX-L组下降更为明显(P<0.05)。病理显示OVA组小鼠肺脏可见严重肺泡腔/血管周围炎细胞浸润,部分支气管/细支气管重度增厚。MX-H组、MX-L组和Dex组,肺脏主要可见轻度至中度肺泡腔/血管周围炎细胞浸润。经造模后,OVA组小鼠与Con组小鼠比较,肺组织TLR4、MyD88、NF-κB mRNA及蛋白表达显著升高(P<0.05);经药物治疗后,Dex组、MX-L组、MX-H组小鼠肺组织TLR4、MyD88、NF-κB mRNA及蛋白表达明显下降,其中,MX-H组比MX-L组下降更为明显(P<0.05)。结论:麻杏石甘汤能够改善哮喘小鼠的临床症状,降低气道炎症水平,其作用机制可能与抑制TLR4/MyD88/NF-κB信号通路相关。Objective This study aims to investigate the mechanism of Maxing Shigan Decoction in the treatment of asthma,focusing on the TLR4/MyD88/NF-κB signaling pathway.Method Fifty male BALB/c mice were randomly assigned to five groups,each containing ten mice:a control group(Con group),a model group(OVA group),high and low dose Maxing Shigan Decoction groups(MX-H group,MX-L group),and a dexamethasone group(Dex group).An asthma model was induced through intraperitoneal injection and atomization of ovalbumin.From days 22 to 28,the MX-H and MX-L groups received Maxing Shigan Decoction at doses of 12 mg/kg and 6 mg/kg,respectively,via gavage one hour prior to atomization.The Dex group was administered dexamethasone(0.5 mg/kg)by gavage,while the Con group received an equivalent volume of normal saline.Following the completion of the experiment,the mice were sacrificed.The levels of IL-1βand IL-6 in serum and alveolar lavage fluid were measured using ELISA.Histopathological changes in lung tissue were assessed through HE staining.RT-qPCR was employed to evaluate the mRNA expression of TLR4,MyD88,and NF-κB in the lung tissue of the mice,while Western Blot analysis was conducted to determine the protein expression of TLR4,MyD88,and NF-κB.Result After modeling,the concentrations of IL-1βand IL-6 in both serum and alveolar lavage fluid were significantly elevated in the OVA group compared to the Con group.Following treatment,the concentrations of these cytokines in the Dex,MX-L,and MX-H groups were notably lower than those in the OVA group,with the MX-H group demonstrating a more pronounced reduction compared to the MX-L group(P<0.05).Pathological examination revealed severe alveolar and perivascular inflammatory cell infiltration in the lungs of the OVA group,accompanied by significant thickening of some bronchi and bronchioles.In contrast,mild tomoderate inflammatory cell infiltration was primarily observed in the lungs of the MX-H,MX-L,and Dex groups.After modeling,the expression levels of TLR4,MyD88,and NF-κB mRNA and p

关 键 词：麻杏石甘汤 哮喘 气道炎症 TLR4 

分 类 号：R285.5[医药卫生—中药学]