基于PHBV固态碳源的反硝化性能和生物群落结构  

Denitrification performance and biological community structure based on PHBV solid carbon source

作　　者：王玉明 杨旸 尹淑燕 江心白 王艺碹 刘晓东[1] 沈锦优[1] Wang Yuming;Yang Yang;Yin Shuyan;Jiang Xinbai;Wang Yixuan;Liu Xiaodong;Shen Jinyou(School of Environmental and Biological Engineering,Nanjing University of Science and Technology,Nanjing 210094,China)

机构地区：[1]南京理工大学环境与生物工程学院,江苏南京210094

出　　处：《南京理工大学学报》2025年第1期86-91,132,共7页Journal of Nanjing University of Science and Technology

基　　金：国家重点研发计划(2021YFA1201704)。

摘　　要：以固态碳源聚(3-羟基丁酸酯-co-3-羟基戊酸酯)(PHBV)为填料搭建反硝化生物滤池,探究其在高浓度NO_(3)^(-)-N条件下的反硝化性能和微生物群落结构。结果表明,当初始NO_(3)^(-)-N质量浓度为50 mg/L时,反应器可在4 d内快速启动,且在NO_(3)^(-)-N质量浓度提升阶段(50~200 mg/L),系统表现出较强的抗冲击负荷能力。当NO_(3)^(-)-N提升至较高质量浓度(250 mg/L)时,系统相对稳定阶段NO_(3)^(-)-N和总氮去除率均大于93.0%,平均氮去除速率达到0.476 kg/(m^(3)·d)。高通量测序结果显示,反硝化菌Thauera和固碳降解功能菌Acidovorax等的富集,保证了高浓度NO_(3)^(-)-N条件下系统高效稳定的脱氮性能。同时由PICRUSt 2功能预测分析得,经过高浓度NO_(3)^(-)-N的驯化,反硝化相关基因高度富集。A denitrification biofilter was constructed with solid carbon source poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)as filler,to investigate its denitrification performance and microbial community structure under high NO_(3)^(-)-N concentration.The results showed that when the initial NO_(3)^(-)-N mass concentration was 50 mg/L,the reactor could rapidly start-up within 4 days,and the system showed strong impact load resistance with increased NO_(3)^(-)-N mass concentration(50~200 mg/L).When NO_(3)^(-)-N was increased to a higher mass concentration(250 mg/L),the NO_(3)^(-)-N and total nitrogen removal efficiencies in the relatively stable stage of the system were greater than 93.0%,and the average nitrogen removal rate reached 0.476 kg/(m^(3)·d).High-throughput sequencing results showed that the denitrifying bacteria Thauera and solid carbon source degrading functional bacteria Acidovorax were enriched,which ensured the efficient and stable denitrification performance of the system under high NO_(3)^(-)-N concentration.Meanwhile,according to PICRUSt 2 functional prediction analysis,the results revealed that the denitrification-related genes were highly enriched after the domestication of high concentration of NO_(3)^(-)-N.

关 键 词：固态碳源 高浓度硝酸盐 反硝化 微生物群落结构 

分 类 号：X703.1[环境科学与工程—环境工程]