功法静力性训练通过PINK1/Parkin通路调控T2DM小鼠骨骼肌线粒体自噬的作用机制  

The Mechanism of Gongfa Static Training Regulating Mitophagy in Skeletal Muscle of Type 2 Diabetes Mellitus(T2DM)Mice via the PINK1/Parkin Pathway

作　　者：甘丽祯 吴霞 陈沛 章志 陈哲炜 韦庆波 吴云川[1] GAN Lizhen;WU Xia;CHEN Pei;ZHANG Zhi;CHEN Zhewei;WEI Qingbo;WU Yunchuan(School of Acupuncture-Moxibustion and Tuina,School of Health Preservation and Rehabilitation,Nanjing University of Chinese Medicine,Nanjing 210023,China;Faculty of Chinese Medicine,Macao University of Science and Technology,Macao 999078,China)

机构地区：[1]南京中医药大学针灸推拿学院·养生康复学院,江苏南京210023 [2]澳门科技大学中医药学院,中国澳门999078

出　　处：《南京中医药大学学报》2025年第2期151-159,共9页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家体育总局健身气功管理中心科技攻关项目(QG2023006);南京中医药大学中医学优势学科四期项目开放课题;江苏省中医药科技发展计划重点项目(ZD202303)。

摘　　要：目的探讨功法静力性训练通过PTEN诱导的激酶1(PINK1)/帕金蛋白(Parkin)通路调控线粒体自噬在2型糖尿病(T2DM)骨骼肌胰岛素抵抗中的作用机制。方法采用高脂饮食联合链脲佐菌素(STZ)腹腔注射建立T2DM小鼠模型,随机分为模型组、二甲双胍组、有氧运动组和功法静力性训练组。通过检测血糖、胰岛素抵抗指数(HOMA-IR)、糖化血红蛋白(HbA1c)、脂质代谢指标以及腓肠肌线粒体功能和PINK1、Parkin相关基因和蛋白表达,评估功法静力性训练的干预效果。结果功法静力性训练显著降低了T2DM小鼠的FBG、糖化血红蛋白及胰岛素抵抗指数,改善了脂质代谢,并增强了胰岛素敏感性,通过上调PINK1、Parkin的mRNA和蛋白表达,改善腓肠肌线粒体的结构和功能。结论功法静力性训练通过调控PINK1/Parkin通路,改善了T2DM小鼠骨骼肌线粒体功能和胰岛素抵抗。OBJECTIVE To explore the mechanism by which Gongfa Static Training regulates mitophagy through the PTEN-induced kinase 1(PINK1)/Parkin pathway in skeletal muscle insulin resistance in type 2 diabetes mellitus(T2DM).METHODS T2DM mouse model was established using a high-fat diet combined with streptozotocin(STZ)intraperitoneal injection.Mice were randomly divided into a model group,a metformin group,an aerobic exercise group,and a Gongfa Static Training group.The intervention effects of Gongfa Static Training were evaluated by measuring fasting blood glucose,Homeostatic Model Assessment of Insulin Resistance(HOMA-IR),glycated hemoglobin(HbA1c),lipid metabolism indicators,mitochondrial function in the gastrocnemius muscle,and the expression of PINK1 and Parkin-related genes and proteins.RESULTS Gongfa Static Training significantly reduced fasting blood glucose,HbA1c,and insulin resistance index in T2DM mice,improved lipid metabolism,and enhanced insulin sensitivity.It improved the structure and function of mitochondria in the gastrocnemius muscle by upregulating the mRNA and protein expression of PINK1 and Parkin.CONCLUSION Gongfa Static Training improves mitochondrial function and insulin resistance in the skeletal muscle of T2DM mice by regulating the PINK1/Parkin pathway.

关 键 词：功法静力性训练 线粒体自噬 T2DM HOMA-IR HbA1c PINK1 PARKIN 

分 类 号：R285.5[医药卫生—中药学]