简化薄层色谱法在白花泡桐叶鉴别中的应用  

Application of Simplified Thin-Layer Chromatography in the Analysis of Paulownia fortunei Leaves

作　　者：李明[1] 王清荣 李雨虹 郭洪伟 龙国清 吕双 魏华[1,4,5] LI Ming;WANG Qingrong;LI Yuhong;GUO Hongwei;LONG Guoqing;LYU Shuang;WEI Hua(School of Pharmaceutical Sciences,Jishou University,Jishou 416000,Hunan China;College of Biology and Environmental Science,Jishou University,Jishou 416000,Hunan China;Xiangxi Natural Biotechnology Co.,LTD,Jishou 416000,Hunan China;Tujia Medicine Research Center in Hunan,Jishou 416000,Hunan China;Tujia Medicine Inheritance and Innovation Key Laboratory of the State Ethnic Affairs Commission,Jishou 416000,Hunan China)

机构地区：[1]吉首大学药学院,湖南吉首416000 [2]吉首大学生物资源与环境科学学院,湖南吉首416000 [3]湘西自然生物科技有限公司,湖南吉首416000 [4]湖南省土家医药研究中心,湖南吉首416000 [5]土家医药传承与创新国家民委重点实验室,湖南吉首416000

出　　处：《吉首大学学报(自然科学版)》2024年第6期62-67,共6页Journal of Jishou University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(81960774);湖南省研究生科研创新项目(CX20240933);2022年大学生创新训练计划项目。

摘　　要：建立了一种用于鉴别白花泡桐叶、兰考泡桐叶、毛泡桐叶、台湾泡桐叶的简化一维薄层色谱法.先采用单因素试验筛选出最优二维多次展开薄层色谱法,结合柱层析色谱法对提取液进行分段,再运用一维薄层色谱法展开分段浓缩液,对4种泡桐属植物叶片进行鉴别.结果表明,在二维多次展开中发现了白花泡桐叶的特征性成分斑点;在分段浓缩后的一维薄层色谱中,在紫外光灯(254 nm)下观察,4种泡桐叶存在4个共同特征斑点,在紫外光灯(365 nm)下观察,4种泡桐叶存在7个共同斑点,在显色后日光灯下观察,4种泡桐叶存在12个共同斑点,白花泡桐叶与兰考泡桐叶、毛泡桐叶、台湾泡桐叶差异点共3个,分别是第1段小极性样品R_f值在0.5左右的位置有1个微黄色斑点,第3段大极性样品R_f值在0~0.2的位置有2个淡粉红色斑点.A simplified one-dimensional thin-layer chromatography(TLC)method has been established for the identification of Paulownia species leaves,including Paulownia fortunei,Paulownia elongata,Paulownia tomentosa and Paulownia kawakamii.A single-factor experiment was conducted to select the optimal two-dimensional multiple development TLC method,which was then combined with column chromatography to segment the extract.Then,the one-dimensional TLC method was employed to identify the leaves of the four Paulownia species after developing the concentrated segments.The results showed that in the two-dimensional multiple expansion,characteristic spots of Paulownia fortunei were identified.Additionally,in the one-dimensional thin layer after segmented concentration,four common characteristic spots were observed for the four Paulownia species at 254 nm,seven common spots at 365 nm,and twelve common spots after color reaction under sunlight.The differences between Paulownia fortunei and the other three species were noted:on the developed TLC plate after color reaction,the first segment of the small polar sample showed a slightly yellow spot at an R f value of 0.5,while the third segment of the large polar sample exhibited two light pink spots at an R f value of approximately 0~0.2.

关 键 词：二维薄层 色谱鉴别 白花泡桐叶 质量控制 

分 类 号：R286.0[医药卫生—中药学]