球药隔重楼根茎与延龄草根茎及重楼的薄层色谱鉴别  

Thin-Layer Chromatography Identification of Paris fargesii Rhizome,Trillium tschonoskii Rhizome,and Paris Rhizome

作　　者：杨德泉 佘永红 YANG Dequan;SHE Yonghong(Food and Drug Inspection Institute,Xiangxi Tujia and Miao Autonomous Prefecture,Jishou 416000,Hunan China)

机构地区：[1]湘西土家族苗族自治州食品药品检验所,湖南吉首416000

出　　处：《吉首大学学报(自然科学版)》2024年第6期68-71,共4页Journal of Jishou University(Natural Sciences Edition)

基　　金：湖南省自然科学基金资助项目(2022JJ80032)。

摘　　要：以重楼皂苷II,VI,H为对照品,采用薄层色谱方法,对球药隔重楼根茎、延龄草根茎和重楼(七叶一枝花和云南重楼的根茎)进行了鉴别.结果表明,采用硅胶G薄层板,以三氯甲烷-甲醇-水(体积比15∶5∶1)作为展开剂,在20℃±2℃、湿度50%~60%条件下,3种对照品分离度高,斑点清晰,并根据成分差异,能对球药隔重楼根茎、延龄草根茎和重楼进行薄层色谱鉴别.Paris Saponin II,VI,H were used as reference substances,and the rhizomes of Paris fargesii,Trillium tschonoskii,and Paris(The rhizome of Paris polyphylla Smith var.chinensis(Franch.)Hara and Paris polyphylla Smith var.yunnanensis(Franch.)Hand.-Mazz.)were identified by thin-layer chromatography(TLC).The results showed that the three reference substances had high resolution and clear spots on the silica gel G thin-layer plate with chloroform-methanol-water(15∶5∶1)as the developing agent under the conditions of 20℃±2℃and 50%~60%.According to the difference in components,TLC can be used to distinguish Paridis fargesii rhizome,Trillium tschonoskii rhizome,and Paris rhizome.

关 键 词：球药隔重楼根茎 延龄草根茎 重楼 重楼皂苷Ⅱ 重楼皂苷Ⅵ 重楼皂苷H 薄层色谱鉴别 

分 类 号：R917[医药卫生—药物分析学]