血塞通胶囊(三七总皂苷)对皮肤冻伤小鼠缺血/再灌注损伤的影响  

Effects of Xuesaitong Capsules(Panax notoginseng saponins)on ischemia/reperfusion injury in a mouse model of skin frostbite

作　　者：秦成 孔祥一 廖晨希 李润丽 王芳 杨雪松 叶建州 QIN Cheng;KONG Xiang-yi;LIAO Chen-xi;LI Run-li;WANG Fang;YANG Xue-song;YE Jian-zhou(The First College of Clinical Medicine,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;Department of Dermatology,The First Hospital Affiliated to Yunnan University of Traditional Chinese Medicine,Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,China)

机构地区：[1]云南中医药大学第一临床医学院,云南昆明650500 [2]云南中医药大学第一附属医院,云南省中医医院皮肤科,云南昆明650021

出　　处：《中成药》2025年第2期430-437,共8页Chinese Traditional Patent Medicine

基　　金：云南民族大学民族药资源化学国家民族事务委员会—教育部重点实验室开放基金项目(MZY2202);云南省中医(皮肤病)临床医学中心[云卫办医发(2021)24号]。

摘　　要：目的探讨血塞通胶囊(三七总皂苷)对皮肤冻伤小鼠缺血/再灌注损伤的作用。方法小鼠随机分为空白组、模型组、地塞米松组(1 mg/kg)和血塞通胶囊低、中、高剂量组(0.036、0.072、0.144 g/kg),每组8只,采用干冰冷冻陶瓷(铁氧体)磁铁造模法建立皮肤冻伤小鼠模型。造模第2天,各组灌胃给予相应剂量药物,连续14 d。观察各组小鼠伤口愈合情况、组织病理学改变;ELISA法检测血清超敏C反应蛋白(hs-CRP)、血栓素(TX)B2、6-醛前列环素(6-K-PGF)1α、一氧化氮(NO)、内皮素(ET)水平;生化检测皮肤组织中超氧化物歧化酶(SOD)活性及丙二醇(MDA)水平;Western blot法检测皮肤组织肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-6、Toll样受体(TLR)4、磷酸化核转录因子(p-NF)-κB p65蛋白表达;RT-qPCR法检测皮肤组织长链非编码RNA(LncRNA)H 19 mRNA表达。结果末次给药后,与空白组比较,模型组小鼠创面痂皮部分脱落,创面愈合,伤口面积较大;组织病理学表现为表皮角化过度伴角化不全,真皮层与皮下组织剥脱游离,附属器部分缺失,皮下软组织疏松、水肿,间质血管扩张、充血、出血,大量淋巴细胞浸润;血清hs-CRP、TXB2、ET水平升高(P<0.05,P<0.01),6-K-PGF1α、NO水平降低(P<0.05,P<0.01);皮肤组织SOD活性降低(P<0.01),MDA水平升高(P<0.01);皮肤组织TNF-α、IL-1β、IL-6、TLR4、p-NF-κB p65蛋白表达升高(P<0.05,P<0.01);皮肤组织LncRNA H 19 mRNA表达升高(P<0.01)。与模型组比较,高剂量血塞通胶囊干预可缩小创面面积(P<0.01);降低血清hs-CRP、TXB2、ET水平(P<0.05,P<0.01),升高6-K-PGF1a、NO水平(P<0.05,P<0.01);升高皮肤组织SOD活性(P<0.05,P<0.01),降低MDA水平(P<0.05,P<0.01);降低皮肤组织TNF-α、IL-1β、IL-6、TLR4、p-NF-κB p65蛋白表达(P<0.05,P<0.01);降低皮肤组织LncRNA H 19 mRNA表达(P<0.05,P<0.01)。结论血塞通胶囊可以通过抗炎、抗氧化应激损伤、恢复血管内皮功能等减少小鼠皮肤AIM To investigate the effects of Xuesaitong Capsules(Panax notoginseng saponins)on ischemia/reperfusion injury in a mouse model of skin frostbite.METHODS The mice were randomly divided into the control group,the model group,the dexamethasone group(1 mg/kg),and the low-dose,medium-dose,and high-dose Xuesaitong Capsules groups(0.036,0.072,and 0.144 g/kg),with eight mice in each group.A frostbite model was established using a dry ice-cooled ceramic(ferrite)magnet.On the 2nd day after modeling,each group started its corresponding dosing by gavage for 14 consecutive days.The wound healing,histopathological changes,and serum levels of high-sensitivity C-reactive protein(hs-CRP),thromboxane B2(TXB2),6-keto-prostaglandin F1α(6-K-PGF1α),nitric oxide(NO)and endothelin(ET)were assessed using ELISA.The superoxide dismutase(SOD)activity and malondialdehyde(MDA)levels in skin tissues were measured biochemically.The protein expressions of tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6,Toll-like receptor(TLR)4 and phosphorylated nuclear factor-κB p65(p-NF-κB p65)in skin tissues were determined by Western blot.Additionally,LncRNA H 19 mRNA expression in skin tissues was evaluated using RT-qPCR.RESULTS After the final administration,compared with the control group,the model group exhibited partial scab detachment,wound healing,and larger wound areas;hyperkeratosis with incomplete keratinization,detachment of the dermis and subcutaneous tissue,partial loss of appendages,subcutaneous edema,and dilated,congested,and hemorrhagic stromal vessels with extensive lymphocyte infiltration revealed by the histopathological examination;elevated serum levels of hs-CRP,TXB2,and ET(P<0.05,P<0.01);decreased 6-K-PGF1αand NO levels(P<0.05,P<0.01);reduced SOD activity in skin tissues(P<0.01);increased MDA levels(P<0.01);and upregulated protein expressions of TNF-α,IL-1β,IL-6,TLR4 and p-NF-κB p65,as well as LncRNA H 19 mRNA expression(P<0.05,P<0.01).Compared with the model group,the group intervened with high-dose Xuesaitong Capsules

关 键 词：血塞通胶囊 三七总皂苷 冻伤 缺血/再灌注损伤 LncRNA H 19 TLR4/NF-κB信号通路 炎症 氧化应激 

分 类 号：R285.5[医药卫生—中药学]