交泰丸对高脂饮食诱导肥胖小鼠下丘脑炎症的改善作用  

Effects of Jiaotai Pills on high-fat diet-induced hypothalamic inflammation in obese mice

作　　者：王晖 袁琳[1] 胡娜[1] 林敏[1] 姜逸 陆敏[1] 王啸南 陆雄[1] 钟霄毓[1] WANG Hui;YUAN Lin;HU Na;LIN Min;JIANG Yi;LU Min;WANG Xiao-nan;LU Xiong;ZHONG Xiao-yu(Laboratory of Cell Biology and Histopathology,Science and Technology Experimental Center,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学科技实验中心细胞生物与组织病理学实验室,上海201203

出　　处：《中成药》2025年第2期446-452,共7页Chinese Traditional Patent Medicine

基　　金：上海中医药大学预算内项目(2021LK038)。

摘　　要：目的研究交泰丸及单味药对高脂饮食诱导肥胖小鼠下丘脑炎症的影响。方法将C57BL/6J小鼠随机分为正常组(15只)与高脂组(75只),高脂饮食造模12周后随机分为模型组、交泰丸组、黄连组、肉桂组、二甲双胍组,每组15只。给药6周后检测小鼠体质量及空腹血糖(FBG)水平,RT-qPCR法检测下丘脑IL-1β、IL-6、TNF-α、Socs 3 mRNA表达,Western blot法检测下丘脑TLR4、MyD88、IKKβ和活化NF-κB蛋白表达,免疫组化法检测下丘脑Iba1、GFAP表达,透射电镜观察神经组织的超微结构变化。结果与模型组比较,各用药组下丘脑IL-1β、IL-6、TNF-α、Socs 3 mRNA表达降低(P<0.01),神经细胞在透射电镜下数量和形态上有所改善,交泰丸组、黄连组和二甲双胍组小鼠体质量和FBG水平降低(P<0.05),TLR4、MyD88、IKKβ、p-NF-κB蛋白表达均降低(P<0.05),下丘脑星形胶质细胞和小胶质细胞减少(P<0.05),肉桂组p-NF-κB蛋白表达降低(P<0.05)。结论交泰丸及其单味药均具有一定改善高脂饮食诱导肥胖小鼠下丘脑炎症的作用。AIM To study the effects of Jiaotai Pills and their single composition drugs on high-fat diet-induced hypothalamic inflammation in obese mice.METHODS C57BL/6J mice were randomly divided into the normal group(15 mice)and the high-fat group(75 mice).The mice given 12 weeks of high-fat diet feeding were further randomly divided into the model group,the Jiaotai Pills group,the Coptis chinensis group,the Cinnamomum cassia group and the positive metformin group,with 15 mice in each group.After 6 weeks of administration,the mice had their body weight and fasting blood glucose(FBG)levels detected;their hypothalamic expressions of IL-1β,IL-6,TNF-αand Socs 3 mRNA detected by RT-qPCR;their hypothalamic expressions of TLR4,MyD88,IKKβand activated NF-κB protein detected by Western blot;their hypothalamic expressions of Iba1 and GFAP detected by immunohistochemistry;and their ultrastructural changes of nerve tissues observed using transmission electron microscopy(TEM).RESULTS Compared with the model group,each drug group displayed decreased hypothalamic expressions of IL-1β,IL-6,TNF-αand Socs 3 mRNA(P<0.01),and improved number and morphology of nerve cells revealed by TEM.The groups intervened with Jiaotai Pills,or Coptis chinensis,or metformin shared decreased body weight and FBG levels(P<0.05);decreased protein expressions of TLR4,MyD88,IKKβand p-NF-κB(P<0.05);and decreased number of hypothalamic astrocytes and microglia(P<0.05).Additionally,decreased p-NF-κB protein expression was observed in the Cinnamomum cassia group(P<0.05).CONCLUSION Jiaotai Pills and their single composition drugs can improve high-fat diet-induced hypothalamic inflammation in obese mice.

关 键 词：交泰丸 下丘脑 炎症 神经胶质细胞 TLR4/NF-κB信号通路 

分 类 号：R285.5[医药卫生—中药学]