美丽石斛茎叶化学成分及其生物活性研究  

Chemical constituents from the stems and leaves of Dendrobium formosum and their biological activities

作　　者：迟利航 陈惠琴[2] 黄圣卓[2] 吴妃 梅文莉[2] 宋希强 戴好富[2] CHI Li-hang;CHEN Hui-qin;HUANG Sheng-zhuo;WU Fei;MEI Wen-li;SONG Xi-qiang;DAI Hao-fu(Key Laboratory of Genetics and Germ plasm Innovation of Tropical Special Forest Trees and Ornamental Plant,Ministry of Education,College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China;Key Laboratory of Natural Products Research and Development of Li Folk Medicine of Hainan Province,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]海南大学热带农林学院热带特色林木花卉遗传与种质创新教育部重点实验室,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南省黎药资源天然产物研究与利用重点实验室,海南海口571101

出　　处：《中成药》2025年第2期467-473,共7页Chinese Traditional Patent Medicine

基　　金：国家中药材产业技术体系专项项目(CARS-21);海南省院士创新平台科研项目(YSPTZX202037)。

摘　　要：目的研究美丽石斛Dendrobium formosum Roxb.ex Lindl.茎叶的化学成分及其生物活性。方法美丽石斛茎叶95%乙醇提取物采用硅胶、Sephadex LH-20及半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用PNPG法测定其α-葡萄糖苷酶抑制活性,RAW264.7模型评价其体外抗炎活性。结果从中分离得到15个化合物,分别鉴定为coniferyl p-coumarate(1)、(-)-松脂醇(2)、2,5,7-三羟基-4-甲氧基-9,10-二氢菲(3)、柚皮素(4)、spiropreussomerin A(5)、7-hydroxy-14-de-O-methyl-lasiodiplodin(6)、(4 S,5S,6Z,8E)-5-hydroxydeca-6,8-dien-4-olide(7)、(6 S,9R)-blumenol C(8)、对羟基苯甲酸(9)、间羟基苯甲酸(10)、对羟基苯丙酸(11)、5,7-二羟基-异苯并呋喃酮(12)、2-(4-羟基苯基)-乙醇(13)、β-谷甾酮(14)、β-谷甾醇(15)。化合物1、4抑制α-葡萄糖苷酶的IC 50值分别为(65.60±3.31)、(98.95±2.53)μmol/L;化合物3可抑制RAW264.7细胞产生NO,IC 50值为(3.97±0.12)μmol/L。结论化合物5~6、8、12为首次从兰科植物中分离得到,2~15为首次从该植物中分离得到。化合物1、4具有α-葡萄糖苷酶抑制活性,3具有抗炎活性。AIM To study the chemical constituents from the stems and leaves of Dendrobium formosum Roxb.ex Lindl.and their biological activities.METHODS The 95%ethanol extract from the stems and leaves of D.formosum was isolated and purified by silica gel,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their inhibitory activities onα-glucosidase were determined by PNPG method,and their in vitro anti-inflammatory activities were evaluated by RAW264.7 model.RESULTS Fifteen compounds were isolated and identified as coniferyl p-coumarate(1),(-)-pinoresinol(2),2,5,7-trihydroxy-4-methoxy-9,10-dihydrophenanthrene(3),naringenin(4),spiropreussomerin A(5),7-hydroxy-14-de-O-methyl-lasiodiplodin(6),(4 S,5S,6Z,8E)-5-hydroxydeca-6,8-dien-4-olide(7),(6 S,9R)-blumenol C(8),p-hydroxybenzoic acid(9),m-hydroxybenzoic acid(10),p-hydroxy benzenepropanoic acid(11),5,7-dihydroxy-isobenzofuran(12),2-(4-hydroxyphenyl)-ethanol(13),β-sitostenone(14),β-sitosterol(15).The IC 50 values of compounds 1 and 4 onα-glucosidase inhibition were(65.60±3.31)and(98.95±2.53)μmol/L,respectively.Compound 3 presented inhibitory activity on NO production in RAW 264.7 cells,with IC 50 value of(3.97±0.12)μmol/L.CONCLUSION Compounds 5-6,8 and 12 are isolated from Orchidacae family for the first time,and 2-15 are first isolated from this plant.Compounds 1 and 4 haveα-glucosidase inhibitory activities,and 3 has anti-inflammatory activity.

关 键 词：美丽石斛 茎叶 化学成分 分离鉴定 α-葡萄糖苷酶抑制活性 体外抗炎活性 PNPG法 RAW264.7细胞 

分 类 号：R284.1[医药卫生—中药学]