紫菀化学成分及其抗炎活性研究  

Chemical constituents from Asteris Radix et Rhizoma and their anti-inflammatory activities

作　　者：李佩 赵付霞 杨思琦 谭金燕 王颖莉 程艳刚 LI Pei;ZHAO Fu-xia;YANG Si-qi;TAN Jin-yan;WANG Ying-li;CHENG Yan-gang(Shanxi University of Chinese Medicine,Jinzhong 030619,China)

机构地区：[1]山西中医药大学,山西晋中030619

出　　处：《中成药》2025年第2期473-479,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82404840);山西省基础研究计划自由探索类青年项目(20210302123309);山西省优秀博士毕业生来晋工作奖励经费科研启动基金项目(2022BKS07);山西省科技创新人才团队专项计划(202204051002028);山西省研究生科研创新项目(2023KY686);山西中医药大学学科建设项目(2023XKJS-26);山西中医药大学科技创新团队(2022TD1015);山西中医药大学研究生教育改革及创新创业项目(2023CX042)。

摘　　要：目的研究紫菀的化学成分及其抗炎活性。方法紫菀提取物采用柱色谱及高效液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用RAW264.7模型评价其抗炎活性。结果从中分离得到13个化合物,分别鉴定为(Z)-9,10,11-三羟基-12-十八碳烯酸(1)、天师酸(2)、6,6-dimethyl-2-methlenebicyclo[3.1.1]hept-3-O-(6-O-apiofuranosyl)-β-D-glucopyranoside(3)、对映-16β,17-二羟基贝壳杉烷-19-羧酸(4)、对映-17-羟基贝壳杉烷-19-羧酸(5)、7β,17-dihydroxy-16α-ent-kauran-19-oic acid 19-O-β-D-glucopyranoside ester(6)、paniculosideⅣ(7)、thomimarine A(8)、cyclo-(S-Pro-R-Leu)(9)、4,5-di-O-caffeoylquinic acid 1-methyl ether(10)、3,5-二咖啡酰基奎宁酸甲酯(11)、5-acetyl-3β-hydroxy-2β-(1-hydroxyisopropyl)-2,3-dihydrobenzofurane(12)、4-烯丙基-2,6-二甲氧基苯基葡萄糖苷(13)。化合物1、3~12对RAW264.7细胞释放NO具有抑制作用,其中4~6、8、10~12优于阳性对照。结论化合物1、6、8~9为首次从菊科分离得到,2~5、7、10、11~13为首次从该植物中分离得到。化合物1、3~12具有抗炎活性。AIM To study the chemical constituents from Asteris Radix et Rhizoma and their anti-inflammatory activities.METHODS The extract from Asteris Radix et Rhizoma was isolated and purified by column chromatography and high performance liquid chromatography,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities were evaluated by RAW264.7 model.RESULTS Thirteen compounds were isolated and identified as(Z)-9,10,11-trihydroxy-12-octadecenoic acid(1),tianshic acid(2),6,6-dimethyl-2-methlenebicyclo[3.1.1]hept-3-O-(6-O-apiofuranosyl)-β-D-glucopyranoside(3),ent-16β,17-dihydroxy-kauran-19-oic acid(4),ent-17-hydroxy-19-kauranoic acid(5),7β,17-dihydroxy-16α-ent-kauran-19-oic acid 19-O-β-D-glucopyranoside ester(6),paniculosideⅣ(7),thomimarine A(8),cyclo-(S-Pro-R-Leu)(9),4,5-di-O-caffeoylquinic acid 1-methyl ether(10),methyl 3,5-di-O-caffeoyl quinate(11),5-acetyl-3β-hydroxy-2β-(1-hydroxyisopropyl)-2,3-dihydrobenzofurane(12),4-ally-2,6-dimethoxyphenyl glucoside(13).Compounds 1 and 3-12 had inhibition on the release of NO in RAW264.7 cells,and 4-6,8,10-12 were better than the positive control.CONCLUSION Compounds 1,6,8-9 are isolated from Compositae family for the first time,and 2-5,7,10 and 11-13 are first isolated from this plant.Compounds 1,3-12 have anti-inflammatory activities.

关 键 词：紫菀 化学成分 分离鉴定 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]