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作 者:何治政 宋金艳 尚巧霞[2] 周莹[1] HE Zhizheng;SONG Jinyan;SHANG Qiaoxia;ZHOU Ying(Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China,Key Laboratory of Environment Friendly Management on Fruit and Vegetable Pests in North China(Co-Construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;College of Bioscience and Resource Environment,Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs,Beijing University of Agriculture,Beijing 102206,China)
机构地区:[1]农业农村部北方果蔬有害生物绿色防控重点实验室(部省共建),北方果树病虫害绿色防控北京市重点实验室,北京市农林科学院植物保护研究所 [2]北京农学院生物与资源环境学院,农业农村部华北都市农业重点实验室
出 处:《北京农学院学报》2025年第1期25-30,共6页Journal of Beijing University of Agriculture
基 金:北京农林科学院创新能力建设项目(KJCX20230810)。
摘 要:【目的】明确从桃树上分离到的壳囊孢属(Cytospora)真菌的生物学特性,探索该属真菌在实验室培养适宜条件,并为该病的防治提供理论依据。【方法】将壳囊孢菌接种至不同培养基上,置于不同温度、pH值、光照、碳氮源条件下,观察记录培养性状。【结果】从桃上分离到的壳囊孢菌在10~35℃均能生长;pH值对壳囊孢菌生长速度的影响较小,在pH值为5~9时,6种壳囊孢菌生长较快,无显著差异;除C.erumpens的最适氮源为牛肉膏外,其他5个种均为酵母膏;6个种的最适碳源均为可溶性淀粉,除C.ailanthicola外均差异显著。在PDA上,C.leucosperma生长速度最快,C.gansuensis最慢。【结论】壳囊孢菌生长最适温度为25~30℃,最适pH值5~9,最佳碳源是可溶性淀粉,最佳氮源为酵母膏。[Objective]To clarify the biological characteristics of Cytospora isolated from peach trees,and to explore the suitable conditions for laboratory cultivation of Cytospora and provide theoretical basis for the prevention and control of this disease.[Methods]Inoculate Cytospora fungi onto different culture media,place them under different temperature,pH,light,carbon and nitrogen source conditions,observe and record the culture characteristics.[Results]Cytospora isolated from peaches can grow at 10-35℃;The effect of pH value on the growth rate of Cytospora is relatively low,the six species of Cytospora in this study grew fast at pH values of 5-9,with no significant difference;the optimal nitrogen source of C.erumpens is beef extract,and the other 5 species are yeast extract;the optimal carbon sources for the 6 species of Cytospora are soluble starch,with significant differences among them except for C.ailanthicola.On PDA,C.leucosperma grows fastest,while C.gansuensis grows slowest.[Conclusion]The optimum temperature was 25-30℃,the optimum pH value was 5-9,the best carbon source was soluble starch,and the best nitrogen source was yeast paste.
分 类 号:S432.1[农业科学—植物病理学]
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