桂附散对猪繁殖与呼吸综合征病毒的抑制作用  

Mechanism of GuiFuSan against porcine reproductive and respiratory syndrome virus based on network pharmacology

作　　者：常可欣 刘博文 周波 吴琼 张华 崔德凤[2] 刘岩琪 宋嘉芸 张永红[2] 童津津 CHANG Kexin;LIU Bowen;ZHOU Bo;WU Qiong;ZHANG Hua;CUI Defeng;LIU Yanqi;SONG Jiayun;ZHANG Yonghong;Tong Jinjin(Beijing Center for Animal Disease Control and Prevention,Beijing 102600,China;College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China;Animal Husbandry and Veterinary Station of Beijing Sanyuan Group,Beijing 100192,China;China Animal Husbandry Industry Co.,LTD,Beijing100070,China)

机构地区：[1]北京市动物疫病预防控制中心,北京102600 [2]北京农学院动物科学技术学院,北京102206 [3]北京三元集团畜牧兽医总站,北京100192 [4]中牧实业股份有限公司,北京100070

出　　处：《北京农学院学报》2025年第1期62-67,共6页Journal of Beijing University of Agriculture

基　　金：北京市农业农村局生猪产业创新团队项目(BAIC02);北京市农业农村局家畜创新团队项目(BAIC05-2022)。

摘　　要：【目的】基于网络药理学探讨桂附散(GFS)对猪繁殖与呼吸综合征病毒(PRRSV)的抑制作用。【方法】通过TCMSP、HERB和ETCM数据库筛选GFS的有效成分及作用靶点,结合CTD、OMIM、GeneCards数据库查找PRRSV的相关靶点,通过STRING数据库构建蛋白互作(PPI)网络;运用Cytoscape 3.7.2软件筛选出核心靶点,导入DAVID数据库进行基因本体(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析,并构建药物-疾病-靶点-通路网络。【结果】结果表明,GFS中发挥抗PRRSV的活性成分有槲皮素、美迪紫檀素、山柰酚、刺芒柄花素、5-甲基-7-甲氧基异黄酮等共136个,核心作用靶点有肿瘤坏死因子、核转录激活蛋白1、细胞肿瘤抗原p53、胱天蛋白酶3和丝裂原激活蛋白激酶14,GO功能和KEGG通路富集分析显示,靶点作用包含生物过程190条、细胞组分14条和分子功能36条,参与趋化因子信号通路、黏着斑、生长激素合成分泌和作用、坏死性凋亡等代谢通路。【结论】综上所述,通过筛选GFS抑制PRRSV的有效成分、关键作用靶点及代谢通路,为GFS抗PRRSV的应用提供了参考依据。[Objective]To explore the mechanism of Guifusan(GFS)in treating porcine reproductive and respiratory syndrome virus(PRRSV)based on network pharmacology in this study.[Methods]Using TCMSP,HERB,and ETCM databases to screen drug component targets,searching for PRRSV related targets through CTD,OMIM,and GeneCards databases,and uploading it to the STRING database to establish a protein interaction(PPI)network;using Cytoscape 3.7.2 software to identify its core targets,using the DAVID database to conduct GO function and KEGG pathway enrichment analysis on core targets,and construct a drug-disease-target-pathway network.[Results]The results of network pharmacology showed that there were 136 active ingredients in GFS for PRRSV,such as quercetin,medallin,kaempferol,formononetin,5-methyl-7-methoxyisoflavone,etc,the core targets of GFS were TNF,JUN,TP53,CASP3 and MAPK14,which were mainly used for chemokine signaling pathway,adhesive spot,growth hormone synthesis,secretion and function,necrotic apoptosis,etc.[Conclusion]In summary,this study screened the active ingredients,key targets and metabolic pathways of GFS for PRRSV,which provided a reference for the application of GFS in the treatment of PRRSV.

关 键 词：桂附散 猪繁殖与呼吸综合征病毒 网络药理学 

分 类 号：S854.4[农业科学—临床兽医学]