槲树UDP-葡萄糖焦磷酸化酶基因的克隆与表达分析  

Cloning and expression of UDP-glucose pyrophosphorylase gene from Quercus dentata

作　　者：武翔悦 白晓宁 蒋萌 梁诗雨 冯思凯 冷平生[1] 赵亚洲[1] 胡增辉[1,2] WU Xiangyue;BAI Xiaoning;JIANG Meng;LIANG Shiyu;FENG Sikai;LENG Pingsheng;ZHAO Yazhou;HU Zenghui(National Forestry and Grassland Ancient Tree Health and Ancient Tree Culture Engineering and Technology Research Center/College of Landscape Architecture,Beijing University of Agricultural,Beijing 102206,China;Beijing Laboratory of Urban and Rural Ecology and Environment,Beijing 102206,China;Beijing Forestry University,Beijing 100091,China;Beijing Meijinglvdu Landscape and Greening Engineering Co.,Ltd,Beijing,102488,China)

机构地区：[1]国家林业草原古树健康与古树文化工程技术研究中心/北京农学院园林学院,北京102206 [2]城乡生态环境北京实验室,北京102206 [3]北京林业大学林学院,北京100091 [4]北京美景绿都园林绿化工程有限公司,北京102488

出　　处：《北京农学院学报》2025年第1期93-98,共6页Journal of Beijing University of Agriculture

基　　金：北京市教委生态修复工程学高精尖学科建设项目。

摘　　要：【目的】土壤盐渍化是限制农林业生产的主要逆境之一。植物能够通过提高可溶性糖含量来抵御盐胁迫,而UDP-葡萄糖焦磷酸化酶(UGP)在可溶性糖代谢中起着重要作用。本研究旨在筛选槲树(Quercus dentata)的UGP基因,进行克隆和表达分析,以判断其对槲树耐盐性的影响。【方法】通过转录组分析筛选出QdUGP2基因,克隆其全长序列,利用qRT-PCR技术检测其在不同部位的表达和对盐胁迫的响应。【结果】QdUGP2全长1428 bp,编码475个氨基酸,其编码蛋白为稳定亲水性蛋白,定位于细胞质,与预测相符。多序列比对显示,QdUGP2蛋白与其他植物UGP蛋白高度相似,尤其是与栎属植物加州白橡木(Q.lobata)同源性最高,达到99.37%。qRT-PCR分析表明,QdUGP2在叶片中的表达量最高,在种子中的表达量最低。在盐胁迫条件下,叶片中的QdUGP2表达呈下降趋势。【结论】槲树QdUGP2参与了槲树对盐胁迫的响应过程。本研究为进一步理解QdUGP2在槲树抗逆性中的功能和作用机制奠定了基础。[Objective]Soil salinization is one of the main stresses limiting agricultural and forestry production.Plants can resist salt stress through increasing the soluble sugar content,and UDP-glucose pyrophosphorylase(UGP)plays an important role in soluble sugar metabolism.In this study,the UGP gene of Quercus dentata was screened,cloned,and analyzed to determine its effect on salt tolerance.[Methods]QdUGP2 was selected based on transcriptome data.The full-length sequence of QdUGP2 was cloned,and its expression levels in different parts and in response to salt stress by qRT-PCR.[Results]The full length of QdUGP2 was 1428 bp,encoding 475 amino acids.The protein encoded by QdUGP2 was a stable hydrophilic protein and located in the cytoplasm,which was consistent with the prediction.Multiple sequence alignment results showed that QdUGP2 was highly consistent with UGP proteins in other plants,and had the highest consistency with the Q.lobata,which was as high as 99.37%.The qRT-PCR analysis showed that QdUGP2 expressed at the highest level in leaves and at the lowest level in seeds.Upon salt stress,the expression of QdUGP2 in leaves showed a downward trend.[Conclusion]QdUGP2 participated in the respond of Q.dentata to salt stress.This study laid a foundation to further understand the function and mechanism of QdUGP2 in the stress resistance of Q.dentata.

关 键 词：槲树 盐胁迫 UDP-葡萄糖合成基因 克隆 表达分析 

分 类 号：Q792.182[生物学—分子生物学] Q786