Determination of Fungal Species to Investigate the Aflatoxin Contamination in Rice(Oryza sativa L.)  

作　　者：Eman Alhomaidi Aisha Umar Mustansar Mubeen Laurent Dufossé Yasir Iftikhar Arpita Das Soumya Ghosh Muhammad Sibt-e-Abbas 

机构地区：[1]Department of Biology,College of Science,Princess Nourah bint Abdulrahman University,P.O.Box 84428,Riyadh,11671,Saudi Arabia [2]Institute of Botany,University of the Punjab,Lahore,54000,Pakistan [3]Department of Plant Pathology,College of Agriculture,University of Sargodha,Sargodha,40100,Pakistan [4]Universitéde La Réunion,Laboratoire CHEMBIOPRO(Chimie et Biotechnologie des Produits Naturels),ESIROI Département agroalimentaire,15 avenue RenéCassin,Saint-Denis,F-97490,France [5]Department of Biotechnology,Adamas University,Barasat-Barrackpore Road,24 Parganas North,Jagannathpur,Kolkata,700126,West Bengal,India [6]Natural and Medical Sciences Research Center,University of Nizwa,Nizwa,616,Oman [7]Department of Food Science&Technology,Faculty of Food&Home Sciences,MNS-University of Agriculture,Multan,630496,Pakistan

出　　处：《Phyton-International Journal of Experimental Botany》2025年第2期407-420,共14页国际实验植物学杂志(英文)

基　　金：supported by Princess Nourah bint Abdulrahman University Researchers Supporting Project Number(PNURSP2025R317),Princess Nourah bintAbdulrahman University,Riyadh,Saudi Arabia.

摘　　要：Aspergillus species produce aflatoxins and raise concerns about food safety in departmental stores and manufacturing mills.To address the risks posed by aflatoxins,and to advise the public on the highest quality rice that serves as a nutritious food source,an inquiry following the guidelines outlined in both local and international standards of food safety for the presence of aflatoxins is an essential requirement.Therefore,16 white rice samples were selected randomly from low/high socio-economic departmental stores from 16 different localities.Grind powdered rice filtrate was extracted using chloroform.The filtrate applied on TLC plates and the amount of aflatoxin and moisture contents were determined.In the non-infected rice,moisture content was low(9.08%)whereas high[13.65%>12%(standard>value)]in infected ones.Four out of 8 samples of low-quality rice were contaminated with AFB_(1) and AFB_(2)(ranging from 22.2 to 29.3μg/kg).All the samples except one(22.3μg/kg)from high-quality rice were certified fit despite the contamination with AFB_(1).Furthermore,phylogenetic analysis showed Aspergillus flavus from unfit low(Long grain brown and Brown basmati)and high-quality(Basmati-198)rice whereas A.parasiticus from unfit low-quality Medium-grain brown rice.The presented research proves that the detection of fungi and aflatoxins in rice grains poses a huge risk to the health of consumers.Therefore,it is necessary to check the rice grains before distribution.

关 键 词：MYCOTOXIN AFLATOXIN RICE contamination TLC principal component analysis 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]