GC-MS Profiling,In Vitro and In Silico Antibacterial and Antioxidant Potential of Origanum elongatum Essential Oil:Novel Source against Phytopathogenic Bacteria  

作　　者：Amine Batbat Mohamed El Fadili Naoufal El Hachlafi Amine Elbouzidi Chaymae Hmimen Hiba Yahyaoui Samir Jeddi Abdellatif Benbouazza Kawtar Fikri-Benbrahim Mohamed Addi Samiah Hamad Al-Mijalli Khaoula Habbadi Hassane Greche 

机构地区：[1]Laboratory of Applied Organic Chemistry,Faculty of Sciences and Techniques,Sidi Mohamed Ben Abdellah University,Imouzzer Road,Fez,30000,Morocco [2]Plant Protection Research Unit,National Institute of Agronomic Research,Regional Center of Agronomic Research of Meknes INRA-CRRA,Meknes,50000,Morocco [3]LIMAS Laboratory,Faculty of Sciences Dhar El Mahraz,Sidi Mohamed Ben Abdellah University,Fez,30000,Morocco [4]Faculty of Medicine and Pharmacy,Ibn Zohr University,Guelmim,81000,Morocco [5]Laboratoire d’Amélioration des Productions Agricoles,Biotechnologie et Environnement(LAPABE),Facultédes Sciences,Univer-sitéMohammed Premier,Oujda,60000,Morocco [6]Laboratory of Microbial Biotechnology and Bioactive Molecules,Faculty of Sciences and Technologies Faculty,Sidi Mohamed Ben Abdellah University,Imouzzer Road,Fez,P.O.Box 2202,Morocco [7]Laboratory of Biotechnology and Bio-Resources Valorization,Faculty of Sciences,Moulay Ismail University,Meknes,50000,Morocco [8]Department of Biology,College of Sciences,Princess Nourah Bint Abdulrahman University,Riyadh,11671,Saudi Arabia

出　　处：《Phyton-International Journal of Experimental Botany》2025年第2期481-501,共21页国际实验植物学杂志(英文)

摘　　要：This study highlights the regulatory potential antibacterial and antiradical of Origanum elongatum essential oil(EO),an endemic medicinal plant of Morocco used for its various properties.The chemical composition of the EO was characterized using gas chromatography-mass spectrometry(GC-MS).The antibacterial activity against different agricultural phytopathogens was determined by disc diffusion and microatmosphere methods,as well as by the determination of minimum inhibitory concentrations(MIC)and minimum bactericidal concentration(MBC),while the antioxidant activity was evaluated by DPPH and FRAP assays.To complement the experimental analyses,a molecular docking approach was used to predict and elucidate the mechanisms of action of the identified bioactive com-pounds,both for their antioxidant and antibacterial properties.The GC-MS analysis revealed a chemical composition dominated by the major compounds:p-cymene-2-ol(25.31%),thymol(23.88%),andγ-terpinene(19.26%).Furthermore,antibacterial analyses performed using different methodological approaches(disc diffusion,microatmosphere,MIC,and MBC)showed significant inhibitory activity against all phytopathogens tested.Moreover,O.elongatum EO exhibited interesting antioxidant ability with an IC_(50) value of 168.251.14μg/mL for DPPH assay and EC_(50) value±of 164.221.04μg/mL for FRAP assay.Furthermore,in silico molecular docking demonstrated further insights into±the interactions between the oil’s active components and bacterial targets,supporting its mode of action.This in-depth characterization highlights the potential of O.elongatum EO as a natural alternative for the biocontrol of plant pathogens.It opens new perspectives for developing natural solutions to protect crops against plant diseases.

关 键 词：Origanum elongatum medicinal plant chemical composition biological activity antiradical activity molecular docking 

分 类 号：R28[医药卫生—中药学]